Invertebrate Neuroscience

, Volume 9, Issue 3–4, pp 195–200 | Cite as

The effect of opioids and their antagonists on the nocifensive response of Caenorhabditis elegans to noxious thermal stimuli

  • F. Nieto-Fernandez
  • S. Andrieux
  • S. Idrees
  • C. Bagnall
  • S. C. Pryor
  • R. Sood
Original Paper

Abstract

Opiates modulate nociception in vertebrates. This has also been demonstrated in a number of invertebrate models. Herein, the effect of the opiate morphine and opioid neuropeptides Endomorphin 1 and 2 on the thermal avoidance (Tav) behavior of Caenorhabditis elegans is explored. Adult wild-type C. elegans N2 were collected from NGM plates using M9 buffer and exposed to morphine and endomorphine 1 and 2 in concentrations between 10−8 and 10−4 M (2.5 pmol/mg to 25 nmol/mg) for 30 min and tested for Tav. The opioid receptor antagonists Naloxone and CTOP were tested in combination with the drugs. Forty-seven percentage of the morphine exposed worms exhibited a class I response versus 76% of the control group (P < 0.001). Endomorphin 1 and 2 also caused a statistically significant reduction in class I responses, 36 and 39%, respectively. These effects were reversed with Naloxone and CTOP. Thermonocifensive behavior in C. elegans is modulated by opioids.

Keywords

Opioid Nematode Nociception 

Notes

Acknowledgments

This work was supported by grants from the NIMH (COR 17138 and MRISP 47392) and the Research Foundation and Central Administration of the State University of New York.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • F. Nieto-Fernandez
    • 1
  • S. Andrieux
    • 1
  • S. Idrees
    • 1
  • C. Bagnall
    • 1
  • S. C. Pryor
    • 1
  • R. Sood
    • 1
  1. 1.SUNY College at Old WestburyOld WestburyUSA

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