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Invertebrate Neuroscience

, Volume 7, Issue 3, pp 173–178 | Cite as

Actions of snake neurotoxins on an insect nicotinic cholinergic synapse

  • Bernard Hue
  • Steven D. Buckingham
  • David Buckingham
  • David B. Sattelle
Original Paper

Abstract

Here we examine the actions of six snake neurotoxins (α-cobratoxin from Naja naja siamensis, erabutoxin-a and b from Laticauda semifasciata; CM12 from N. haje annulifera, toxin III 4 from Notechis scutatus and a long toxin from N. haje) on nicotinic acetylcholine receptors in the cercal afferent, giant interneurone 2 synapse of the cockroach, Periplaneta americana. All toxins tested reduced responses to directly-applied ACh as well as EPSPs evoked by electrical stimulation of nerve XI with similar time courses, suggesting that their action is postsynaptic. Thus, these nicotinic receptors in a well-characterized insect synapse are senstive to both long and short chain neurotoxins. This considerably expands the range of snake toxins that block insect nicotinic acetylcholine receptors and may enable further pharmacological distinctions between nAChR subtypes.

Keywords

Snake neurotoxins Nicotinic acetylcholine receptors Insect central nervous system 

Notes

Acknowledgments

The authors are indebted to Prof. R. E. Hider, Pharmaceutical Science Research Division, 5th floor Franklin-Wilkins Building, King’s College London, 150 Stamford Street, London SE1 9NH for the generous provision of the snake toxin samples used in this study.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Bernard Hue
    • 1
  • Steven D. Buckingham
    • 2
  • David Buckingham
    • 2
  • David B. Sattelle
    • 2
  1. 1.Laboratoire de Physiologie, Faculté de MédécineUniversité d’AngersAngers CedexFrance
  2. 2.MRC Functional Genetics Unit, Department of Physiology Anatomy and GeneticsUniversity of OxfordOxfordUK

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