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Invertebrate Neuroscience

, Volume 7, Issue 2, pp 87–98 | Cite as

Eleven new putative aminergic G-protein coupled receptors from Amphioxus (Branchiostoma floridae): identification, sequence analysis and phylogenetic relationship

  • Chloe Burman
  • Braudel Maqueira
  • John Coadwell
  • Peter D. EvansEmail author
Original Paper

Abstract

We have identified eleven novel aminergic-like G-protein coupled receptor (GPCRs) sequences (named AmphiAmR1-11) by searching the genomic trace sequence database for the amphioxus species, Branchiostoma floridae. They share many of the structural motifs that have been used to characterize vertebrate and invertebrate aminergic GPCRs. A preliminary classification of these receptors has been carried out using both BLAST and Hidden Markov Model analyses. The amphioxus genome appears to express a number of D1-like dopamine receptor sequences, including one related to insect dopamine receptors. It also expresses a number of receptors that resemble invertebrate octopamine/tyramine receptors and others that resemble vertebrate α-adrenergic receptors. Amphioxus also expresses receptors that resemble vertebrate histamine receptors. Several of the novel receptor sequences have been identified in amphioxus cDNA libraries from a number of tissues.

Keywords

Amphioxus Branchistoma floridae G-protein coupled receptor Monoamines Dopamine Octopamine Histamine 

Notes

Acknowledgments

This work was supported by the BBSRC through the Babraham Institute. We thank Dr. Mikhail Matz, Whitney Laboratory, University of Florida, St Augustine, USA for kindly supplying us with the amphioxus cDNA libraries used in this study.

Supplementary material

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Chloe Burman
    • 1
  • Braudel Maqueira
    • 1
  • John Coadwell
    • 2
  • Peter D. Evans
    • 1
    Email author
  1. 1.The Inositide LaboratoryThe Babraham InstituteCambridgeUK
  2. 2.The Bioinformatics SectionThe Babraham InstituteCambridgeUK

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