Invertebrate Neuroscience

, Volume 5, Issue 1, pp 29–35 | Cite as

Action of nereistoxin on recombinant neuronal nicotinic acetylcholine receptors expressed in Xenopus laevis oocytes

  • Valérie Raymond Delpech
  • Makoto Ihara
  • Claudio Coddou
  • Kazuhiko Matsuda
  • David B. Sattelle
Original Articles

Abstract

Nereistoxin (NTX), a natural neurotoxin from the salivary glands of the marine annelid worm Lumbriconereis heteropoda, is highly toxic to insects. Its synthetic analogue, Cartap, was the first commercial insecticide based on a natural product. We have used voltage-clamp electrophysiology to compare the actions of NTX on recombinant nicotinic acetylcholine receptors (nicotinic AChRs) expressed in Xenopus laevis oocytes following nuclear injection of cDNAs. The recombinant nicotinic AChRs investigated were chicken α7, chicken α4β2 and the Drosophila melanogaster/chicken hybrid receptors SAD/β2 and ALS/β2. No agonist action of NTX (0.1–100 μM) was observed on chicken α7, chicken α4β2 and the Drosophila/chicken hybrid nicotinic AChRs. Currents elicited by ACh were reduced in amplitude by NTX in a dose-dependent manner. The toxin was slightly more potent on recombinant Drosophila/vertebrate hybrid receptors than on vertebrate homomeric (α7) or heteromeric (α4β2) nicotinic AChRs. Block by NTX of the chicken α7, chicken α4β2 and the SAD/β2 and ALS/β2 Drosophila/chicken hybrid receptors is in all cases non-competitive. Thus, the site of action on nicotinic AChRs of NTX, to which the insecticide Cartap is metabolised in insects, differs from that of the major nicotinic AChR-active insecticide, imidacloprid.

Keywords

Nicotinic acetylcholine receptor Nereistoxin Chicken α7 subunit Chicken α4β2 receptor Drosophila melanogaster Nicotinic receptor subunits (SAD, ALS) Xenopus laevis oocytes 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Valérie Raymond Delpech
    • 1
    • 4
  • Makoto Ihara
    • 2
  • Claudio Coddou
    • 1
    • 3
  • Kazuhiko Matsuda
    • 2
  • David B. Sattelle
    • 1
  1. 1.MRC Functional Genetics Unit, Department of Human Anatomy and GeneticsUniversity of OxfordOxfordUK
  2. 2.Department of Agricultural Chemistry, Faculty of AgricultureKinki UniversityNaraJapan
  3. 3.Centro de Regulacion Celular y Patalogia, Instituto Milenio MIFAB, Unidad de Regulacion Neurohumoral, Departamento de Ciencias FisiologicasP. Universidad Catolica de ChileCasillaChile
  4. 4.Centre de Recherches sur la Cognition Animale, UMR 5169Université Paul SabatierToulouse CedexFrance

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