Comparison of two fluid solutions for resuscitation in a rabbit model of crush syndrome
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Crush syndrome is a common injury, the main characteristics of which include acute kidney injury. However, there is still lack of reliable animal model of crush syndrome, and it also remains controversial as to which type of fluid should be chosen as a more appropriate treatment option for prevention and treatment of acute kidney injury.
The rabbits were crushed at the lower limbs for 6 h with 36 times the body weight, which means the pressure of each leg was also 36 times the body weight. Fluid resuscitation was performed from 1 h prior to the end of the crush treatment until 24 h after the reperfusion. Tissue, blood and urine samples were collected at predetermined time points before and after reperfusion. Twelve rabbits in each group were taken for survival observation for 72 h.
The model group showed elevated serum creatine kinase, aspartate aminotransferase, alanine aminotransferase, and K+ level, reduced serum Ca2+ level and Na+ level, and increased serum creatinine and blood urea nitrogen levels, neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1 (p < 0.05). The 0.9 % normal saline (SAL) group and SAL plus 6 % hydroxyethyl starch 130/0.4 SAL/HES group showed reduced serum creatinine and blood urea nitrogen levels (p < 0.05). The SAL/HES group also showed reduced serum IL-6 and IL-10 levels (p < 0.05). The 72 h survival rate of the SAL/HES group was higher than that of the model group (p < 0.05).
The rabbit model of crush syndrome showed clinical features consistent with those of crush syndrome. There was no significant difference in the ability of preventing AKI after a crush injury between the two fluid solutions, while SAL/HES can improve the survival rate.
KeywordsCrush syndrome Fluid resuscitation Inflammatory response Oxidative stress
This work was partially supported by a research grant from Chinese PLA 12th Five-Year Plan for Medical Sciences (BWS11J027), a grant from National Natural Science Foundation of China (81170643), and a grant from National Key Technology R&D Program (2011BAI10B00).
Conflict of interest
The authors declare no conflict of interest.
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