Clinical and Experimental Nephrology

, Volume 18, Issue 3, pp 424–431 | Cite as

Effect of a recombinant manganese superoxide dismutase on prevention of contrast-induced acute kidney injury

  • Antonio Pisani
  • Massimo Sabbatini
  • Eleonora RiccioEmail author
  • Roberta Rossano
  • Michele Andreucci
  • Clemente Capasso
  • Viviana De Luca
  • Vincenzo Carginale
  • Mariano Bizzarri
  • Antonella Borrelli
  • Antonella Schiattarella
  • Michele Santangelo
  • Aldo Mancini
Original Article



Contrast media (CM)-induced nephropathy (CIN) is an acute deterioration of renal function following administration of CM mediated to a large extent by the increased production of ROS within the kidney. Aim of this study was to evaluate whether a novel isoform of a recombinant Manganese SOD (rMnSOD) could provide an effective protection against CIN; this molecule shares the same ability of physiological SODs in scavenging reactive oxygen species (ROS) but, due to its peculiar properties, enters inside the cells after its administration.


We studied the effects rMnSOD on oxidative damage in a rat model of CIN in uninephrectomized rats, that were randomly assigned to 3 experimental Groups: Group CON, control rats treated with the vehicle of CM, Group HCM, rats treated with CM and Group SOD, rats treated with CM and rMnSOD.


In normal rats, pretreatment with rMnSOD, reduced renal superoxide anion production, induced by the activation of NAPDH oxidase, by 84 % (p < 0.001). In rats of Group HCM, ROS production was almost doubled compared to rat of Group CON (p < 0.01) but returned to normal values in rats of Group SOD, where a significant increase of SOD activity was detected (+16 % vs HCM, p < 0.05). Administration of CM determined a striking fall of GFR in rats of Group HCM (−70 %, p < 0.001 vs CON), greatly blunted in Group SOD (−28 % vs CON, p < 0.01); this was associated with a lower presence of both tubular necrosis and intratubular casts in SOD-treated rats (both p < 0.01 vs Group HCM).


Our data indicate that rMnSOD is able to reduce renal oxidative stress, thus preventing the reduction of GFR and the renal histologic damage that follows CM administration.


Contrast-induced nephropathy Superoxide dismutase Oxygen radicals 



Body weight


Contrast media-induced nephropathy


Contrast media




2′,7′-Dichlorofluorescin diacetate


Glomerular filtration rate


Intensity Fluorescence






Liposarcoma cell line


Recombinant manganese superoxide dismutase


Reactive oxygen species


Superoxide dismutase


Water-soluble tetrazolium salt


Conflict of interest

None of the authors have anything to disclose.


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Copyright information

© Japanese Society of Nephrology 2013

Authors and Affiliations

  • Antonio Pisani
    • 1
  • Massimo Sabbatini
    • 1
  • Eleonora Riccio
    • 1
    Email author
  • Roberta Rossano
    • 1
  • Michele Andreucci
    • 2
  • Clemente Capasso
    • 3
  • Viviana De Luca
    • 3
  • Vincenzo Carginale
    • 3
  • Mariano Bizzarri
    • 4
  • Antonella Borrelli
    • 5
  • Antonella Schiattarella
    • 5
  • Michele Santangelo
    • 6
  • Aldo Mancini
    • 5
  1. 1.Department of NephrologyUniversity Federico II of NaplesNaplesItaly
  2. 2.Department of NephrologyMagna Graecia UniversityCatanzaroItaly
  3. 3.CNR. Institute of Protein Biochemistry (IBP)NaplesItaly
  4. 4.Italian Space Agency, La Sapienza UniversityRomeItaly
  5. 5.National Cancer Institute G. PascaleNaplesItaly
  6. 6.Department of SurgeryUniversity Federico II of NaplesNaplesItaly

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