Clinical and Experimental Nephrology

, Volume 16, Issue 5, pp 679–689 | Cite as

Effects of adipose-derived mesenchymal cells on ischemia–reperfusion injury in kidney

  • Kengo Furuichi
  • Hidemi Shintani
  • Yoshio Sakai
  • Takahiro Ochiya
  • Kouji Matsushima
  • Shuichi Kaneko
  • Takashi Wada
Original Article



Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI.


Adipose-derived mesenchymal cells were isolated from mouse subcutaneous and peritoneal adipose tissue by digestion with collagenase type I. The left renal artery and vein of C57BL/6 mice were clamped for 45 min to induce ischemia and were injected with the adipose-derived mesenchymal cells [1 × 105 cells/0.2 ml phosphate-buffered saline (PBS)] or 0.2 ml PBS via the tail vein on days 0, 1, and 2.


The adipose-derived mesenchymal cells had stem-cell surface markers and multilineage differentiating potentials. Administered adipose-derived mesenchymal cells homed primarily into lung. Interestingly, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration in the injured kidney, accompanied with reduced cytokine and chemokine expression.


Adipose-derived mesenchymal cells can be used as cell-based therapy for ischemic kidney injury.


Acute kidney injury Ischemic kidney injury Adipose-derived mesenchymal cells Chemokines 



The work was financially supported in part by grants-in-aids from the Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government.

Conflict of interest



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Copyright information

© Japanese Society of Nephrology 2012

Authors and Affiliations

  • Kengo Furuichi
    • 1
  • Hidemi Shintani
    • 2
  • Yoshio Sakai
    • 3
  • Takahiro Ochiya
    • 4
  • Kouji Matsushima
    • 5
  • Shuichi Kaneko
    • 2
  • Takashi Wada
    • 3
  1. 1.Division of Blood PurificationKanazawa University HospitalKanazawaJapan
  2. 2.Department of Disease Control and Homeostasis, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health SciencesKanazawa UniversityKanazawaJapan
  3. 3.Department of Laboratory Medicine, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health SciencesKanazawa UniversityKanazawaJapan
  4. 4.Section for Studies on MetastasisNational Cancer Center Research InstituteTokyoJapan
  5. 5.Department of Molecular Preventive Medicine, Graduate School of MedicineThe University of TokyoTokyoJapan

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