Clinical and Experimental Nephrology

, Volume 10, Issue 2, pp 102–110 | Cite as

Mechanism of iodide transport in the rabbit cortical collecting duct

  • Yohkazu Matsushima
  • Shigeaki Muto
  • Junichi Taniguchi
  • Masashi Imai
ORIGINAL ARTICLE
Received:
Accepted:

Abstract

Background

Pendrin, an anion exchanger known to participate in iodide transport in the apical membrane of follicular cells of the thyroid gland, has recently been shown to exist in the apical membrane of the β- and γ-intercalated (β/γ-IC) cells of the cortical collecting duct (CCD). We examined mechanisms of iodide transport in the CCD.

Methods

Rabbit CCD was perfused in vitro, and lumen-to-bath flux coefficients for both 125I− (KI (lb)) and 36Cl− (KCl (lb)) were measured simultaneously. The intracellular pH (pHi) of β/γ-IC cells in the perfused CCD was measured by microscopic fluorometory, by loading 2′,7′-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein tetraacetoxy methylester (BCECF-AM), a fluorescent marker for pHi. The effects on pHi of the replacement of NaCl with Na cyclamate, NaI, or NaBr in the lumen or bath were observed.

Results

KI (lb) was comparable to or slightly higher than KCl (lb). Both iodide and chloride in the lumen caused self- and cross-inhibitions to both fluxes. The addition of 5-nitro-2-(-3-phenylpropylamino)-benzoate (NPPB), a Cl− channel inhibitor, to the bath significantly reduced KCl (lb), but not KI (lb). Replacement of luminal fluid NaCl with Na cyclamate, NaI, or NaBr caused alkalization of pHi, no change in pHi, and slight acidification of pHi, respectively. Replacement of bath NaCl with Na cyclamate, NaI, or NaBr caused alkalization, alkalization, and acidification of pHi, respectively. Luminal NaI prevented the acidification of pHi caused by bath Na cyclamate.

Conclusions

The data are consistent with the model that iodide is transported via the Cl−/HCO3 − exchanger in the apical membrane of β/γ-IC cells and exits the basolateral membrane via an electroneutral transporter that is distinct from the Cl− channel. We could not, however, identify which type of β/γ-IC cell was mainly responsible.

Key words

Iodide transport Pendrin β-/γ-Intercalated cells Cortical collecting duct Cl−/HCO3− exchanger Cl− channel 
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Copyright information

© Japanese Society of Nephrology 2006

Authors and Affiliations

  • Yohkazu Matsushima
    • 1
  • Shigeaki Muto
    • 2
  • Junichi Taniguchi
    • 3
  • Masashi Imai
    • 3
  1. 1.Department of Cardiovascular Dynamics, Research InstituteNational Cardiovascular CenterOsakaJapan
  2. 2.Department of Internal Medicine, Division of NephrologyJichi Medical UniversityTochigiJapan
  3. 3.Department of Pharmacology, Division of Molecular PharmacologyJichi Medical UniversityTochigiJapan

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