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Journal of Infection and Chemotherapy

, Volume 13, Issue 6, pp 390–395 | Cite as

Substrate specificity of HMRZ-86 for β-lactamases, including extended-spectrum β-lactamases (ESBLs)

  • Hideaki Hanaki
  • Yuuji Koide
  • Hiroaki Yamazaki
  • Ryoichi KuboEmail author
  • Tomota Nakano
  • Koichiro Atsuda
  • Keisuke Sunakawa
ORIGINAL ARTICLE

Abstract

HMRZ-86 was designed as a new chromogenic cephalosporin to detect extended-spectrum β-lactamases (ESBLs) and similar evolved β-lactamases, such as metallo-β-lactamases, derepressed AmpC, and extended oxacillinase. We report here our investigation of the kinetic parameters of several types of β-lactamases to show the enzymatic characteristics of HMRZ-86. The Michaelis constant (K m values of HMRZ-86 for ESBLs were twice to three and half times as high as those of nitrocefin, and the maximum velocity (Vmax) was one-fifth that of nitrocefin. The K m and Vmax of HMRZ-86 for AmpC were both smaller than those of nitrocefin. The kinetic parameters of HMRZ-86 for metallo β-lactamase (MBL) were very variable, depending on the type of buffer solution used and the concentration of zinc ions. For MBL, the K m values of HMRZ-86 were higher than those of nitrocefin, but the Vmax values were almost the same as those of nitrocefin. Although the chemical structure of HMRZ-86 is similar to that of nitrocefin, we think the enzymatic reactivities of the two entities for β-lactamases are very different.

Key words

β-Lactamase extended-spectrum β-lactamases (ESBLs) Metallo β-lactamase (MBL) HMRZ-86 Enzymology 

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Copyright information

© Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases 2007

Authors and Affiliations

  • Hideaki Hanaki
    • 1
  • Yuuji Koide
    • 2
  • Hiroaki Yamazaki
    • 2
  • Ryoichi Kubo
    • 3
    Email author
  • Tomota Nakano
    • 4
  • Koichiro Atsuda
    • 5
  • Keisuke Sunakawa
    • 6
  1. 1.Research Center for Anti-Infective DrugsKitasato InstituteTokyoJapan
  2. 2.Research LaboratoryZenyaku Kogyo Co., Ltd.TokyoJapan
  3. 3.Department of Life ScienceKanto Chemical Co., Inc.TokyoJapan
  4. 4.Isehara Research LaboratoryKanagawaJapan
  5. 5.Center for Clinical Pharmacy and Clinical ScienceKitasato UniversityKitasatoJapan
  6. 6.Department of Infectious DiseaseKitasato UniversityKitasatoJapan

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