Neutralization of staphylococcal exotoxins in vitro by human-origin intravenous immunoglobulin

  • Chie YanagisawaEmail author
  • Hideaki Hanaki
  • Taiji Natae
  • Keisuke Sunakawa


Human-origin intravenous immunoglobulin (IVIG) collected from healthy individuals was tested for its neutralizing activity against the hemolysin, toxic shock syndrome toxin-1 (TSST-1), and enterotoxins, produced by laboratory strains of methicillin-resistant Staphylococcus aureus (MRSA). The hemolytic activity of the culture supernatant against sheep red blood cells was reduced from 100% to 5.5% relative hemolysis in the presence of 0.156 mg protein/ml of IVIG. The maximum dilution endpoint of the culture supernatant for TSST-1-mediated latex aggregation was 32-fold. This level of TSST-1 activity was reduced to eightfold dilution by 0.78 mg protein/ml of IVIG, and the latex aggregation activity of undiluted TSST-1 in the culture supernatant was inhibited by 1.56 mg protein/ml of IVIG. Similarly, the enterotoxin A-mediated latex aggregation titer appeared to be a 320-fold dilution. This toxin activity was reduced to an 80-fold dilution and below a tenfold dilution by 0.049 through 0.78 and 1.56 mg protein/ml, respectively, of IVIG. These results show that IVIG has powerful neutralizing activity against hemolysin, TSST-1, and enterotoxin A. Therefore, IVIG may be useful for passive immunization therapy in patients suffering from diseases caused by MRSA exotoxins.

Key words

Methicillin-resistant Staphylococcus aureus (MRSA) Exotoxin Intravenous immunoglobulin (IVIG) 


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Copyright information

© Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases 2007

Authors and Affiliations

  • Chie Yanagisawa
    • 1
    Email author
  • Hideaki Hanaki
    • 1
  • Taiji Natae
    • 1
  • Keisuke Sunakawa
    • 2
  1. 1.Kitasato Research Center for Anti-infection DrugsTokyoJapan
  2. 2.Graduate School of Infection Control Sciences, Kitasato Institute for Life SciencesKitasato UniversityTokyoJapan

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