Biology of Grapsus grapsus (Linnaeus, 1758) (Brachyura, Grapsidae) in the Saint Peter and Saint Paul Archipelago, Equatorial Atlantic Ocean
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Eleven expeditions were undertaken to the Saint Peter and Saint Paul Archipelago to study the reproductive biology of Grapsus grapsus, providing additional information on limb mutilation and carapace colour. MATURE software was used to estimate morphological maturity, while gonadal analyses were conducted to estimate physiological maturity. The puberty moult took place at larger size in males (51.4 mm of carapace length) than in females (33.8 mm), while physiological maturity occurred at a similar size in males (38.4 mm) and in females (33.4 mm). Above 50 mm, the proportion of red males increased in the population, indicating that functional maturity is also related to colour pattern. Small habitat and high local population density contributed to the high rate of cannibalism. The low diversity of food items, absence of predators of large crabs and high geographic isolation are the determinants of unique behavioural and biological characteristics observed in the G. grapsus population.
KeywordsGrapsus grapsus Reproductive biology Sexual maturity Mutilation Equatorial Atlantic Ocean
Due to the installation of a Brazilian scientific station on Saint Paul’s rocks, it was possible to begin a broad investigation into Grapsus grapsus biology since 2003, focusing the research on larval ecology (Koettker et al. 2009), growth, abundance monitoring, intraspecific and interspecific interactions (Gianuca and Vooren 2007) and the present one about reproductive biology.
Determination of the size at which brachyuran crab species begins sexual activity is a key parameter to understanding its life cycle (Hartnoll and Gould 1988). Furthermore, estimation of size of sexual maturity is one of the most important information for planning management programmes (Pinheiro and Fransozo 1998), being marked by several morphological, physiological and behavioural changes, indicating the transition between phases (Hartnoll 1985).
The life cycle of G. grapsus, as with most brachyuran crabs, involves a planktonic larval phase (zoea), recruitment of megalopae and growth through instars. According to Hartnoll (1963), the relative growth of some brachyuran crustacean, like Majoidea crabs, can be characterized by three phases: immature, prepuberal and mature. These phases are separated by two important moults during ontogeny: prepuberty moult, in which immature crabs begin morphologic changes to become adults, and puberty moult, in which crabs acquire other morphological characters to become mature. The transition of the last juvenile instar to the first adult one is marked by a critical moult (the ‘puberty moult’), which is accompanied by morphological changes (Hartnoll 2001). At the puberty moult, male chelae enlarge fast in relation to carapace width to be a better partner’s hold, and females have an enlargement of their abdomen to protect the egg mass. These subtle changes in the relative proportion of these body parts make possible a precise estimative of the size at morphological maturity. Females of various crab species can and do mate (morphological maturity) before the ovaries mature, while males are required to present mature gonads (physiological maturity) (Schejter and Spivak 2005). The size at which morphological and physiological maturity are achieved is not necessarily the same; as a result, a determination of these different sizes at maturity in both sexes can be used to estimate functional maturity (Pinheiro and Fransozo 1998; Pinheiro and Fiscarelli 2001). Functional maturity is a broader concept that should also include mating ability, which depends on physiological, behavioural and morphological aspects (Corgos and Freire 2006). Sexual maturity studies about crabs are very common in the literature, especially in relation to species of economic importance, and also with grapsoids that play a key ecological role in their habitats (e.g. Flores et al.2002; Flores and Paula 2002; Luppi et al. 2004).
The focus of this study is to estimate physiological and morphological maturity sizes of G. grapsus on Saint Paul’s rocks, as well as to describe carapace colour patterns and their possible correlation with maturity. An evaluation of sex ratio and the incidence of mutilated individuals (limb autotomy and carapace injures) in the population are also presented. The findings reported here add new information to the few existing studies on the reproduction investment of tropical crab species (Hartnoll 2006), with possible use at management programmes.
Materials and methods
Crabs were spread out in the whole island, occupying different microhabitats as the rocky pools, rocky coast, rocky crevices, breeding and nesting sites of the booby Sula leucogaster (Boddaert, 1783), under the scientific station and over the pier. The crabs were sampled randomly and manually, in all these microhabitats, using a 50-cm2 net over the rocky floor or directly with the hands. Crabs were sampled during the day, especially in the morning, when the action of the waves was less intense. The crabs were immediately frozen in individual plastic bags and duly labelled. Samples were taken on 11 expeditions from February 2003 to May 2005, with the number of crabs/expedition limited by the Scientific Program.
During the laboratory analyses, the specimens were identified by sex and classified as males, non-ovigerous and ovigerous females. The abdomen shape of the females was classified at three categories (triangular, triangular/round or round) (Fig. 6). The following measurements were taken with a vernier calliper (0.05 mm): carapace width and length (CW and CL, respectively), propodus length of the cheliped (PL), greatest width of the fifth female abdominal somite (AW) and length of first male gonopods (GL). Exemplars were dissected for gonadal development stage estimation by macroscopic analysis.
The relative growth of propodus and gonopod (males) and pleon (females) was studied using log-transformed data (see Pinheiro and Fransozo 1998, for details). The Snedecor’s F test (a = 0.01) was used to check whether one or two regression lines fit better the data. The biometric relations with best fit were used to describe growth changes between phases and estimate morphological maturity size in brachyurans, according to the procedures described by Pinheiro and Fransozo (1998). The data for each biometric relation establish a characteristic relative growth pattern and were processed using specific computer software: MATURE 1 (Somerton 1980) or MATURE 2 (Somerton and Macintosh 1983).
Description of gonadal development stages used in macroscopic analyses of males and females of Grapsus grapus
Not visible. Hepatopancreas highly developed
Not visible. Hepatopancreas highly developed
Small ovaries; light yellow to light orange
Vas deferens clearly visible; translucent to whitish
Ovaries larger; orange to red with distinct oocytes. Ovaries occupying most of the available cephalothorax space, at least 1/2 of the size of the hepatopancreas
Testes convoluted to enlarged and sinuous, white, at least 1/4 of the size of the hepatopancreas
The size at which half the population of individuals are mature (CW50%) was established by size distribution of each sex according to their gonadal maturation stage (IM = immature; DE = developing; MA = mature) into 5-mm CW classes, by plotting the proportion of adult stages (DE + MA) in relation to juveniles (IM) for each CW class. The frequency of adults by size class was fitted for each sex using Galton’s Ogive function (y = 1 − e−AZ, with Z = xb). An estimates of physiological maturation (CW50%) was obtained for each sex by CW50% = e[ln[−ln(1−0,5)]−lnA]/b (Fonteles-Filho 1989).
The morphological and physiological maturity sizes to each sex were compared to verify a possible synchrony between these events and a possible establishment of functional maturity.
The colour pattern of the specimens was categorized into four groups: (1) Green: carapace green/dark green, with light yellow spots (Fig. 8); (2) Red leg: carapace dark green and pereiopods/chelipeds dark red; (3) Red frontal plate: carapace slight dark red (red in the frontal plate) and dark red legs; and (4) Red: carapace fully and bright red, yellow to white lines and spots and red legs (Fig. 1). The relation between colour pattern categories to CW was analysed graphically.
Crabs sampled randomly during 4 expeditions (n = 522) were used to register mutilation (injuries in the carapace as scars, holes, missing pereiopods and regeneration bourse). Frequency distribution of mutilated crabs and number of missing pereiopods were analysed by size (CW) class, and results obtained to each sex were compared by chi-square test (Zar 1998).
Monthly abundance (number of individuals) of each sex and ovigerous condition, as well as monthly sex ratio at Saint Peter and Saint Paul Archipelago, Brazil
Morphological maturity estimation
Results of biometric relations obtained for Grapsus grapsus and allometric level of each equation
Equation (y = axb)
Somerton’s F test*
PL × CW
PL = 0.544CW0.91
PL = 0.214CW1.19
PL = 0.0179CW1.82
GL × CW
GL = 0.0125CW1.94
GL = 0.398CW0.91
AW × CW
AW = 0.119CW1.37
AW = 0.0314CW1.80
AW = 0.777CW0.96
MATURE 1 revealed three regressions for biometric relation PL × CW: CW < 28 mm; 28 ≤ CW < 51.4 mm; and CW ≥ 51.4 mm. There was negative allometric growth before the prepuberty moult (28 mm) followed by positive growth until the puberty moult at 51.4 mm. After the puberty moult, there was a huge increase in the allometric constant (1.19–1.82).
The same software revealed two regression lines for the relation GL × CW, with inflection pointed at 28.8 mm. Changes in the relative growth rates of chelae and gonopods provided almost identical estimates of prepuberty size (28 and 28.8 mm, respectively). It is noticeable that allometric growth was positive before the inflection point (28.8 mm) for the GL × CW relation but negative before the inflection point (28 mm) for the PL × CW relation.
For the females, the relation AW × CW was expressed by three different regressions: (1) CW < 23.8 mm; (2) 23.8 ≤ CW < 39.4 mm; and (3) CW ≥ 33.8 mm. The inflection point between regression lines 1 and 2 (23.8 mm) was considered the prepuberty size, and the positive allometric constant increased from the lower line towards the upper one (MATURE 1). MATURE 2 was used for the 29.9–39.4 mm CW zone, due to overlap between regression lines in this case. The lower line was the upper one from the previous data (b = 1.80), changed to negative allometric growth (0.96) after 33.8 mm. The prepuberty size for morphological maturity was very near between sexes (males, 28.8 mm; females, 23.8 mm), but puberty moult took place at a considerably larger size in males (51.4 mm) than in females (33.8 mm).
Physiological maturity estimation
Among the females, 44% had gonads in the developing stage and 20% had mature gonads. Most of the ovigerous females (77%) had gonads in the developing stage, and none were observed with immature gonads. Among the males, the general proportion of gonadal stages was very similar, with 45% in the developing stage (DE) and 25% having mature gonads (MA). Crabs with mature gonads were found in all months sampled.
Morphology of the female abdomen
Range of CW (carapace width, mm) within each abdomen shape category in Grapsus grapsus females
Number of crabs with missing limbs (from 1 to 4) and register of the missing pereiopods in Grapsus grapsus
No. of missing limbs
R and L
Data about size range of Grapsus grapsus are only available for Pacific Ocean sites. The maximum sizes attained by G. grapsus from SPSPA (males, 69.5 mm; females, 57.3 mm) were smaller than recorded in Galapagos by Veintimilla (1975) (75 mm), in Mexico by Rathbun (1918) (87 mm) and in Peru by Romero (2003) (males, 79.8 mm; females, 74.6 mm). The maximum size of G. grapsus males sampled in this study was similar to G. adscencionalis (67 mm), although the females of the latter species were slight larger (69 mm) (Hartnoll 2009).
In brachyuran crabs, the size at the onset of sexual maturity using a single criterion may lead to unrealistic conclusions regarding the actual size at which these organisms are functionally mature (Flores et al.2002). Lopez Grego and Rodrigues (1999) evidenced a great contrast between different estimates for Chasmagnathus granulatus. In the case of Perisesarma guttatum, gonadal maturity was also reached much earlier than morphological maturity, while the size of the smallest ovigerous female was surprisingly large, closer to maturity size by biometry (Flores et al.2002). Estimates based on the smallest ovigerous crab are dependent on sample size and the ovigerous state and do not be used alone to estimate mature specimens (Flores and Paula 2002).
Females of G. grapsus with three different shapes of abdomen could be found within the range of the morphological maturity size. In grapsoid crabs, when the abdomen reaches the basis of pereiopod coxae (maximum AW), the female is considered morphologically mature; in Chasmagnathus granulatus, at least two moults were necessary to complete morphological maturation of the abdomen (Luppi et al. 2004), with some females observed carrying eggs after first moult, although this sex became morphologically mature when the abdomen is clearly wider and rounded (Luppi et al.2004).
In G. grapsus, the prepuberty size (23.8 mm) was reached concurrently during transition to the round abdominal shape (21.2–24.1 mm). Female crabs from 23.8 to 33.8 mm (with intermediate abdomen shape) could be designated as adolescent (or prepuberal), according to the terminology adopted by Alunno-Bruscia and Sainte-Marie (1998) and Luppi et al. (2004); however, only after 39.4 mm, females were invariably adults.
Only 5.6% of the 90 females with smaller sizes than the overlap between the prepuberal and puberal phases (<31.6 mm) had developing and mature gonads. In the overlap of these two upper regression lines (31.6–36.8 mm) occurs 47 specimens that comprise 19 (40.4%) prepuberal and 28 (59.6%) adults, whereas 74.5% (35 females) had functional maturity. In sizes greater than the overlap (>36.8 mm), only seven females (4.2%) of 165 specimens showed no functional maturity. Ovigerous females were registered since 31.6 mm and comprise a good estimate of the onset of functional maturity of G. grapsus in this study. In Galapagos, most of the G. grapsus ovigerous females ranged from 36 to 45 mm CW (Veintimilla 1975), reinforcing our results for female adult size in this species. Sexual maturity of G. adscencionalis was also reached at a similar size (Hartnoll 2009).
Defining size at the onset of functional maturity is more difficult in males because there is increasing evidence that males bearing fully developed testes may be prevented from mating (Flores and Paula 2002). According to the Flores et al. (2002), the high positive allometric growth of the gonopods can be explained by the distinct development of pleopods into functional gonopods, the same occurring with exemplars of G. grapsus with CW < 28.8 mm. After that size, gonopods growth became negatively allometric; the crabs allocated their energy to increasing their chelae, confirmed by positive allometric growth of propodus length. Chelipeds are used in territorial defence, combat, display and courtship (Hartnoll 1982). The continuous increase in the positive allometric growth of the chelae, even after the 51.4 mm (cut-off point), indicates that enlargement of the chelipeds is essential to functional maturity. The onset of gonadal maturation in males occurs since 24.2 mm, all being morphologically immature until 51.4 mm (size of morphological maturity), with 47.2% of them showing developing and mature gonadal stages. From 51.4 mm, 96.2% of individuals have mature gonads and were morphologically and physiologically suitable for reproduction (functional maturity). Male physiological maturity of G. adscencionalis (32–36 mm), established by Hartnoll (2009), was similar to G. grapsus, and they also exhibited higher allometric growth of chelae among larger crabs.
After 50 mm, the proportion of red males increased in the population, indicating that maturity is also related to this colour pattern change. Male crabs from 38.4 to 51.4 mm had functional gonopods and were physiological mature, but their proportional chelae size made them less able to compete with the red males. In fact, red frontal plate males could be seen copulating in the rocks when the breeding embrace took place far from the fully red males. In grapsoids, size differences between potentially mature males may be quite high and exclusion of juvenile crabs during agonistic interactions is expected (Flores et al.2002). Males were larger than females in all mating pairs observed, which was caused by the size of males at functional maturity being greater than female ones. Mating couples involving a larger male and a smaller female are commonly recorded in brachyuran crabs (e.g. Pinheiro and Fransozo 1999; Flores and Paula 2002), and small males are also under risk of being predated by the female (Lopez Grego and Rodrigues 1999). Cannibalism was observed in a G. grapsus Peruvian population after the copula, resulting in the death of the male (Romero 2003), which was not seen at the site studied by us.
In many marine crabs, phenotypic plasticity in carapace colouration patterns has been observed (Reuschel and Schubart 2007) and may involve some advantage against visual predators (Todd et al. 2005). Colouration patterns in semi-terrestrial crabs may change with growth, seasonality, current state of the individual such as stress, endogenous rhythms and even changes in temperature (Silbiger and Munguia 2008). Carapace colour has been also correlated with respiratory and osmoregulatory physiology, response to desiccation, moult cycle, chelae biometry/prey selection, chelae autotomy and mating behaviour (Reid et al.1997).
The fact that mature Grapsus grapsus males had at least some red colour in their carapace indicated that this colour pattern was important in mating behaviour. Most of the time we observed red leg and red frontal plate male crabs interested in the same female, and the fully red ones could displace them. Colour and size has already been considered representative of hierarchy status in other male grapsoid crabs (reviewed in Abello et al.1997). The existence of relation between colour, size and sex was described in Perisesarma (Huang et al.2007) and Heloecius cordiformis (Detto et al.2004).
In Xanthoporessa, the different colour patterns of juveniles and adults are still controversial. Bedini (2002) suggested that the juveniles have colour patterns that conform to cryptic mimicry in sea grass and that the adults change their colour at their final moult to complete adulthood and move to rocky shores. However, Reuschel and Schubart (2007) argued that both juveniles and adults were found with highly variable colours, suggesting that they use different colours and disruptive cryptic colouration as passive defence (camouflage). In the SPSPA, green-grey G. grapsus were manly found at sea level, on vertical and negative rocky walls, well camouflaged against the dark rocks. On the other hand, the fully red crabs were proportionally more abundant in the rocky pools, nesting bird sites and protected horizontal rocks. Carcinus maenas also had a single colour form dominating in a particular area, but the extensive review of Reid et al. (1997) was unable to elucidate the reason. The change in allometric growth of Grapsus grapsus near to the size at which fully red crabs were proportionally more abundant was related to the fact that larger and red crabs had wider and more circular carapaces than green ones. Changes in allometric growth were also associated with changes to the carapace shape of X. poressa (Reuschel and Schubart 2007).
Females of Grapsus grapsus in Galapagos also changed their colour gradually from black to red as size increased, reinforcing the popular belief that the larger and redder the crabs are, the more mature they are (Veintimilla 1975). In the SPSPA, red males of G. grapsus were more successful in mating, but males with some green in the carapace were also sexually mature. The colour pattern did not seem to be very important for female breeding success, since sexually mature and ovigerous females of all colours were found.
The aggressive intraspecific behaviour of the fully red crabs was even observed when the green crabs were simply walking nearby and not only related to breeding competition. The encounter was usually followed by autotomy and escape or by the death and consumption of the smaller crab. Smaller victims reduce the risk to predators and reduce handling time, making the prey item more profitable in terms of energy returns (Kurihara and Okamoto 1987). Data on injured crabs within a population are very variable in the literature (9–65%) (review by Davis et al. 2005). Cannibalism, expressed by the mutilation index, among G. grapsus in the SPSPA (24%) was much higher than in Peru (5%) (Romero 2003). Cannibalism is a common phenomenon amongst crustaceans and certain conditions encourage this behaviour: an unusual small habitat that prevents dispersal and promotes high population densities (Moller et al. 2008), limited food and little available refuge (Marshall et al. 2005). All of these conditions were present in our site, but we do not know which factors contributed to the high mutilation rate. A high percentage of crabs with only one missing limb, a slightly smaller percentage of missing chela, both sexes equally mutilated and large males with scars were also observed in Peruvian G. grapsus (Romero 2003). The impact of limb losses may indirectly affect other parameters, such as habitat choice and distribution (Davis et al.2005) and the growth and structure of crab populations (Luppi et al. 2001). If a rocky intertidal crab is unable to negotiate its habitat as well with missing walking legs, it may choose an area less influenced by tidal energy until its limbs regenerate (Davis et al. 2005). The negative effects of mutilation on growth have been described in other crab species, and limb regeneration may affect the process of morphological change at the onset of maturity (Luppi et al. 2001). Limb autotomy, especially in species with high incidence of autotomies, should be considered in crab fisheries where size at the onset of maturity is relevant (Luppi et al. 2001).
The environmental conditions at the SPSPA are unique, due to the low diversity of food items, lack of predators for large crabs and high degree of geographic isolation. On other Brazilian oceanic islands, G. grapsus feed on newborn turtles, while on the Peruvian coast the species preys on cirripeds and mytilideans mussels (Romero 2003), all of which are absent at our site. However, the booby Sula leucogaster colony represents an important food source for the crab, contributing to the high abundance of crabs in relation to other sites (Gianuca and Vooren 2007). These features, together with the high mutilation rate, can affect functional maturity size. Future work on reproduction and behaviour on the Brazilian oceanic islands should take account of the contribution of the environment to the definition of maturity. Molecular, morphological, biometric and carapace colour studies will clarify the status of this species in the Atlantic Ocean islands, as well as the connectivity between its populations.
This research was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Grant 48.0040/2004-4 and 55.8470/2005-0) and Comissão Interministerial para os Recursos do Mar (CIRM). We would like to thank the crew of the fishing boats Transmar I and Transmar II and all the biologists, aquaculture engineers and oceanographers who helped during the field work and crab biometry, especially Vanessa Bevilacqua Tomin Borges for initial data sampling and analysis. We would like to thank Luis Carlos P. Macedo-Soares and Matheus M. Coelho (ECZ/UFSC) for the help with the maps and graphs.