Abstract
Background
Although serum p53 autoantibodies (s-p53-Abs) are induced even in the early stages of colorectal cancer, their positive rate is only approximately 20%. Therefore, we assessed the possibility of using other serum autoantibodies to increase the positive rates for detecting colorectal cancer.
Methods
Autoantibodies against 17 tumor antigens (p53, RalA, HSP70, Galectin1, KM-HN-1, NY-ESO-1, p90, Sui1, HSP40, CyclinB1, HCC-22-5, c-myc, PrxVI, VEGF, HCA25a, p62, and Annexin II) were evaluated in 279 patients with colorectal cancer and 74 healthy controls. Cutoff values were fixed at mean + 3 standard deviations of serum titers in healthy controls.
Results
Autoantibodies with the highest positive rates were p53 (20%), RalA (14%), HSP70 (12%), and Galectin1 (11%). Combination assays using multiple autoantibodies increased the positive rates based on the number of autoantibodies used. Positive rates of 56, 62, 66, 71, and 73% were obtained with 6, 9, 11, 14, and 17 antibodies, respectively, for the overall disease. Moreover, these autoantibodies showed relatively high positive rates even during stage 0/I disease (55 and 70% with 6 and 17 antibodies, respectively).
Conclusion
The measurement of set of 17 autoantibodies allowed autoantibody profiling in patients with colorectal cancer. The combination assay of six tumor antigens (p53, RalA, HSP70, Galectin1, KM-HN-1, and NY-ESO-1) achieved a positive rate of 56%. Such high positive rates will be helpful for detecting colorectal cancer regardless of tumor stages.
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Acknowledgements
Junichi Koike received research grant from JSPS KAKENHI Grant Number JP26462029.
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Hideaki Shimada received research Grants from Medical & Biological Laboratories Co., Ltd., Nagoya, Japan. Akiko Kuwajima is an employee of the Medical & Biological Laboratories Co., Ltd., Nagoya, Japan. The other authors declare that there are no conflicts of interest associated with the present study.
Informed consent
Informed consent was obtained from all patients, and the study was approved by the Ethics Committee of Chiba Cancer Center (no. 21-26) and Toho University School of Medicine (nos. 22-112 and 22-047). This clinical study has been registered in the UMIN Clinical Trials Registry (UMIN000014530).
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Ushigome, M., Nabeya, Y., Soda, H. et al. Multi-panel assay of serum autoantibodies in colorectal cancer. Int J Clin Oncol 23, 917–923 (2018). https://doi.org/10.1007/s10147-018-1278-3
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DOI: https://doi.org/10.1007/s10147-018-1278-3