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International Journal of Clinical Oncology

, Volume 10, Issue 2, pp 122–126 | Cite as

Intracellular signaling in the induction of apoptosis in a human breast cancer cell line by water extract of Mekabu

  • Masanori SekiyaEmail author
  • Hiroomi Funahashi
  • Kyosuke Tsukamura
  • Tsuneo Imai
  • Akemi Hayakawa
  • Tetsuya Kiuchi
  • Akimasa Nakao
ORIGINAL ARTICLE

Abstract

Background

We previously reported that water extract of Mekabu, a kind of seaweed, induced apoptosis in a human breast cancer cell line. In the present study we investigated intracellular signaling in apoptosis, with a focus on caspases.

Methods

Mekabu extract, obtained with ultrapure water, was used to induce apoptosis in a human breast cancer cell line, MDA-MB231, and DNA fractionation was investigated by flow cytometry and electrophoresis. In addition, using the caspase detection kit Caspa Tag, activation of caspases 3, 6, 8, and 9 was observed under a fluorescence microscope. Furthermore, using antibodies to caspases 3, 8, 9, and Bid, we conducted a protein analysis by Western blotting to determine the activation of these substances.

Results

Obvious ladder formation demonstrating DNA fractionation was seen, confirming that Mekabu extract induced apoptosis. In the fluorescence microscope observations, activation of caspases 3, 6, and 8, but not caspase 9, was seen. Activated caspases 3 and 8 were detected in the Western blotting analysis, but no proteins of activated caspase 9 or Bid were detected.

Conclusion

Mekabu extract activates caspases 3, 6, and 8 and contributes to intracellular signaling to induce apoptosis in a human breast cancer cell line. This signaling is not via the mitochondria.

Key words

Water extract of Mekabu Breast cancer Apoptosis Caspase Bid 

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Copyright information

© The Japan Society of Clinical Oncology 2005

Authors and Affiliations

  • Masanori Sekiya
    • 1
    Email author
  • Hiroomi Funahashi
    • 2
  • Kyosuke Tsukamura
    • 1
  • Tsuneo Imai
    • 1
  • Akemi Hayakawa
    • 3
  • Tetsuya Kiuchi
    • 1
  • Akimasa Nakao
    • 1
  1. 1.Second Department of SurgeryNagoya University School of MedicineNagoyaJapan
  2. 2.Department of SurgeryGifu Prefectural HospitalGifuJapan
  3. 3.Equipment Center for Research and EducationNagoya University Graduate School of MedicineNagoyaJapan

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