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International Journal of Clinical Oncology

, Volume 9, Issue 3, pp 184–188 | Cite as

Erythropoietin/Erythropoietin-receptor system as an angiogenic factor in chemically induced murine hepatic tumors

  • Kiyoshi NakamatsuEmail author
  • Yasumasa Nishimura
  • Minoru Suzuki
  • Shuichi Kanamori
  • Osamu Maenishi
  • Yoshiko Yasuda
ORIGINAL ARTICLE

Background

To clarify the role of erythropoietin (Epo) in hepatic tumor angiogenesis, expression of Epo and its receptor (Epo-R) and content of Epo were investigated in murine chemically induced hepatic tumors.

Methods

To induce hepatic tumors and cirrhosis, diaminobenzidine was administered to Wistar rats for 5 months. In total, 30 hepatic tumors of greater than 3 mm in diameter were induced in 12 rats. The 30 hepatic tumors were resected with the surrounding hepatic tissues. The Epo content was measured by a radioimmunoassay (RIA) method. The number of tumor vessels in a definite area was counted in 100 areas of each tumor. To demonstrate the expression of Epo-R in tumors or surrounding liver tissues, immunohistochemial staining for Epo-R was performed.

Results

The Epo content of tumors ranged from 6.1 to 97.8 mU/ml, with a median of 21.8 mU/ml, which was significantly higher than that of the cirrhotic tissues adjacent to the tumors. Epo was not detectable in the normal or cirrhotic liver tissues without tumors. A significant correlation between Epo content and vascular density was noted in the 30 hepatic tumors (correlation coefficient, 0.480; P = 0.01). Immunoreactive Epo-R was detectable in the endothelium of intervening vessels of all hepatic tumors examined.

Conclusion

The Epo/Epo-R system is related to the angiogenesis of murine hepatic tumors.

Key words

Erythropoietin Erythropoietin receptor Tumor angiogenesis Hepatic tumors Diaminobenzidine Rats 

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Copyright information

© Springer-Verlag Tokyo 2004

Authors and Affiliations

  • Kiyoshi Nakamatsu
    • 1
    Email author
  • Yasumasa Nishimura
    • 1
  • Minoru Suzuki
    • 1
  • Shuichi Kanamori
    • 1
  • Osamu Maenishi
    • 2
  • Yoshiko Yasuda
    • 3
  1. 1.Department of RadiologyKinki University School of MedicineOsakaJapan
  2. 2.First Department of PathologyKinki University School of MedicineOsakaJapan
  3. 3.First Department of AnatomyKinki University School of MedicineOsakaJapan

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