Functional & Integrative Genomics

, Volume 20, Issue 1, pp 89–101 | Cite as

De novo genome assembly and comparative annotation reveals metabolic versatility in cellulolytic bacteria from cropland and forest soils

  • Suman Yadav
  • Bhaskar Reddy
  • Suresh Kumar DubeyEmail author
Original Article


Cellulose, the most abundant polysaccharide in nature, is a rich source of renewable energy and sustains soil nutrients. Among the microorganisms known to degrade cellulose, bacteria are less studied compared to fungi. In the present work, we have investigated the culturable bacteria actively involved in cellulose degradation in forest and crop field soils. Based on clear zone formation and enzyme activity assay, we identified 7 bacterial strains positive for cellulose degradation. Of these, two most efficient strains (Bacillus cereus strains BHU1 and BHU2) were selected for whole genome sequencing, annotation, and information regarding GC content, number of genes, total subsystems, starch, and cellulose degradation pathways. Average nucleotide identity (ANI) showed more than 90% similarity between both the strains (BHU1 and BHU2) and with B. cereus ATCC 14579. Both the strains have genes and enzyme families like endoglucanase and β-glucosidase as evident from whole genome sequence. Cellulase containing gene families (GH5, GH8, GH1), and many other carbohydrate-degrading enzymes, were present in both the bacterial strains. Taken together, the results suggest that the strains were efficient in cellulose degradation, and can be used for energy generation and production of value-added product.


Crop and forest soils Bacillus cereus Cellulase De novo genome assembly GH family 



We also thank Coordinator CAS, DST-FIST, and PURSE for facilities.

Funding information

One of the authors (Suman) is grateful to CSIR, New Delhi, India, for the financial assistance in the form of Junior and Senior Research Fellowship.

Compliance with ethical standards

Conflict of interest

Authors have no any conflict of interest for this publication

Supplementary material

10142_2019_704_MOESM1_ESM.doc (74 kb)
Fig. S1 Starch and sucrose metabolic pathway of B. cereus BHU1 and BHU2 strain. Green colored boxes show the presence of ECs in both the isolates while yellow color boxes reveal the absence of ECs in BHU2 strain (DOC 74 kb)
10142_2019_704_MOESM2_ESM.xlsx (85 kb)
Table S1 Subsystem and InterPro annotation of B. cereus BHU1 and BHU2 strains (XLSX 84 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Suman Yadav
    • 1
  • Bhaskar Reddy
    • 1
  • Suresh Kumar Dubey
    • 1
    Email author
  1. 1.Laboratory of Molecular Ecology, Centre of Advanced Study in Botany, Institute of ScienceBanaras Hindu UniversityVaranasiIndia

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