Proteomic and transcriptomic analysis to unravel the influence of high temperature on banana fruit during postharvest storage
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Banana, an important food, incurs significant economic losses due to high storage temperature. Integrative analysis of proteome and transcriptome profiles of the banana peel stored at 20 °C (control) and 30 °C (HT) was used to investigate the molecular mechanism in response to high temperature stress. Critical proteins and genes relating to the response of banana fruit to HT stress were evaluated using partial least squares-discriminant analysis (PLS-DA) and orthogonal signal correction partial least squares-discriminant analysis (OPLS-DA). HT stress influenced proteins/genes related to chlorophyll metabolism, fruit firmness, signal transduction, energy metabolism, and stress response and defense. Together with scanning electron microscopy (SEM) and real time quantitative PCR (RT-qPCR) results, it can be concluded that HT stress resulted in stay-green ripening of banana fruit. Additionally, HT stress accelerated firmness loss and senescence of banana peel, might mainly through regulating hormone signaling pathway, stress protective ability, and energy metabolism in the banana peel. Our study provided a clearer understanding of regulatory mechanisms of HT treatment on banana fruit and potential genetic resources for the improvement of high temperature-tolerant characteristics in banana fruit.
KeywordsFruit Temperature Transcriptome Proteome Stress
We extend sincere thanks and gratitude to Afiya John, a native English speaker, for her kind assistance in revision and editing of this manuscript.
This work was supported by Pearl River S&T Nova Program of Guangzhou (No.201610010041), National Natural Science Foundation of China (Grant Nos. 31671911, 31701657), Young Elite Scientists Sponsorship Program by CAST (2017QNRC001), and Key Laboratory of Post-Harvest Handling of fruits, Ministry of Agriculture.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399. https://doi.org/10.1146/annurev.arplant.55.031903.141701 CrossRefGoogle Scholar
- Biyiklioglu S, Alptekin B, Akpinar BA, Varella AC, Hofland ML, Weaver DK, Bothner B, Budak H (2018) A large-scale multiomics analysis of wheat stem solidness and the wheat stem sawfly feeding response, and syntenic associations in barley, Brachypodium, and rice. Funct Integr Genomics 18:241–259. https://doi.org/10.1007/s10142-017-0585-5 CrossRefGoogle Scholar
- Bouzroud S, Gouiaa S, Hu N, Bernadac A, Mila I, Bendaou N, Smouni AA, Bouzayen M, Zouine M (2018) Auxin response factors (ARFs) are potential mediators of auxin action in tomato response to biotic and abiotic stress (Solanum lycopersicum). PLoS One 13:e0193517. https://doi.org/10.1371/journal.pone.0193517 CrossRefGoogle Scholar
- Grassl J, Pruzinska A, Hortensteiner S, Taylor NL, Millar AH (2012) Early events in plastid protein degradation in stay-green Arabidopsis reveal differential regulation beyond the retention of LHCII and chlorophyll. J Proteome Res 11:5443–5452. https://doi.org/10.1021/pr300691k CrossRefGoogle Scholar
- He WD, Gao J, Dou TX, Shao XH, Bi FC, Sheng O, Deng GM, Li CY, Hu CH, Liu JH, Zhang S, Yang QS, Yi GJ (2018) Early cold-induced peroxidases and aquaporins are associated with high cold tolerance in Dajiao (Musa spp. ‘Dajiao’). Front Plant Sci 9:282. https://doi.org/10.3389/fpls.2018.00282 CrossRefGoogle Scholar
- Li L, Luo ZS, Huang XH, Zhang L, Zhao P, Ma H, Li X, Ban Z, Liu X (2015a) Label-free quantitative proteomics to investigate strawberry fruit proteome changes under controlled atmosphere and low temperature storage. J Proteome 120:44–57. https://doi.org/10.1016/j.jprot.2015.02.016 CrossRefGoogle Scholar
- Lin Y, Lin Y, Lin H, Lin M, Li H, Yuan F, Chen Y, Xiao J (2018) Effects of paper containing 1-MCP postharvest treatment on the disassembly of cell wall polysaccharides and softening in Younai plum fruit during storage. Food Chem 264:1–8. https://doi.org/10.1016/j.foodchem.2018.05.031 CrossRefGoogle Scholar
- Mao J, Li W, Mi B, Ma Z, Dawuda MM, Zuo C, Zhang Y, Jiang X, Chen B (2017) Transcriptome analysis revealed glucose application affects plant hormone signal transduction pathway in “Red Globe” grape plantlets. Plant Growth Regul 84:45–56. https://doi.org/10.1007/s10725-017-0320-1 CrossRefGoogle Scholar
- Ortiz R, Braun HJ, Crossa J, Crouch JH, Davenport G, Dixon J, Dreisigacker S, Duveiller E, He ZH, Huerta J, Joshi AK, Kishii M, Kosina P, Manes Y, Mezzalama M, Morgounov A, Murakami J, Nicol J, Ferrara GO, Ortiz-Monasterio JI, Payne TS, Pena RJ, Reynolds MP, Sayre KD, Sharma RC, Singh RP, Wang JK, Warburton M, Wu HX, Iwanaga M (2008) Wheat genetic resources enhancement by the International Maize and Wheat Improvement Center (CIMMYT). Genet Resour Crop Evol 55:1095–1140. https://doi.org/10.1007/s10722-008-9372-4 CrossRefGoogle Scholar
- Page D, Gouble B, Valot B, Bouchet JP, Callot C, Kretzschmar A, Causse M, Renard CMCG, Faurobert M (2010) Protective proteins are differentially expressed in tomato genotypes differing for their tolerance to low-temperature storage. Planta 232:483–500. https://doi.org/10.1007/s00425-010-1184-z CrossRefGoogle Scholar
- Raimbault AK, Zuily-Fodil Y, Soler A, Carvalho MHCD (2013) A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): cloning, characterization and relation to postharvest chilling stress resistance. J Plant Physiol 170:1536–1540. https://doi.org/10.1016/j.jplph.2013.06.007 CrossRefGoogle Scholar
- Sanchez-Bel P, Egea I, Sanchez-Ballesta MT, Sevillano L, Del CBM, Flores FB (2012) Proteome changes in tomato fruits prior to visible symptoms of chilling injury are linked to defensive mechanisms, uncoupling of photosynthetic processes and protein degradation machinery. Plant Cell Physiol 53:470–484. https://doi.org/10.1093/pcp/pcr191 CrossRefGoogle Scholar
- Steingass CB, Jutzi M, Muller J, Carle R, Schmarr HG (2015) Ripening-dependent metabolic changes in the volatiles of pineapple (Ananas comosus (L.) Merr.) fruit: II. Multivariate statistical profiling of pineapple aroma compounds based on comprehensive two-dimensional gas chromatography-mass spectrometry. Anal Bioanal Chem 407:2609–2624. https://doi.org/10.1007/s00216-015-8475-y CrossRefGoogle Scholar
- Wu Q, Zhang Z, Zhu H, Li T, Zhu X, Gao H, Yun Z, Jiang Y (2018) Comparative volatile compounds and primary metabolites profiling of pitaya fruit peel after ozone treatment. J Sci Food Agric. https://doi.org/10.1002/jsfa.9479
- Yun Z, Qu HX, Wang H, Zhu F, Zhang Z, Duan X, Yang B, Cheng Y, Jiang Y (2016) Comparative transcriptome and metabolome provides new insights into the regulatory mechanisms of accelerated senescence in litchi fruit after cold storage. Sci Rep 6:19356. https://doi.org/10.1038/Srep19356 CrossRefGoogle Scholar