Functional & Integrative Genomics

, Volume 16, Issue 4, pp 347–363 | Cite as

A characterization of grapevine of GRAS domain transcription factor gene family

  • Xin Sun
  • Zhengqiang Xie
  • Cheng Zhang
  • Qian Mu
  • Weimin Wu
  • Baoju Wang
  • Jinggui Fang
Original Article
First Online:
Received:
Revised:
Accepted:

Abstract

GRAS domain genes are a group of important plant-specific transcription factors that have been reported to be involved in plant development. In order to know the roles of GRAS genes in grapevine, a widely cultivated fruit crop, the study on grapevine GRAS (VvGRAS) was carried out, and from which, 43 were identified from 12× assemble grapevine genomic sequences. Further, the genomic structures, synteny, phylogeny, expression profiles in different tissues of these genes, and their roles in response to stress were investigated. Among the genes, two potential target genes (VvSCL15 and VvSCL22) for VvmiR171 were experimentally verified by PPM-RACE and RLM-RACE, in that not only the cleavage sites of miR171 on the target mRNA were mapped but also the cleaved fragments and their expressing patterns were detected. Transgenic Arabidopsis plants over expression VvSCL15 showed lower tolerance to drought and salt treatments.

Keywords

Grapevine GRAS gene family Expression Function 
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Supplementary material

10142_2016_479_MOESM1_ESM.doc (172 kb)
Fig S1 (DOC 171 kb)
10142_2016_479_MOESM2_ESM.xls (22 kb)
Table S1 (XLS 21 kb)
10142_2016_479_MOESM3_ESM.doc (92 kb)
Table S2 (DOC 91 kb)
10142_2016_479_MOESM4_ESM.xls (32 kb)
Table S3 (XLS 32 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Xin Sun
    • 1
  • Zhengqiang Xie
    • 1
    • 2
  • Cheng Zhang
    • 1
  • Qian Mu
    • 1
  • Weimin Wu
    • 3
  • Baoju Wang
    • 1
  • Jinggui Fang
    • 1
  1. 1.College of HorticultureNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.Department of Agronomy and HorticultureJiangsu Polytechnic College of Agriculture and ForestryJurongPeople’s Republic of China
  3. 3.Institute of HorticultureJiangsu Academy of Agriculture SciencesNanjingPeople’s Republic of China

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