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Functional & Integrative Genomics

, Volume 14, Issue 1, pp 111–125 | Cite as

The dehydrin wzy2 promoter from wheat defines its contribution to stress tolerance

  • Weining Zhu
  • Linsheng ZhangEmail author
  • Hui Lv
  • Hongmei Zhang
  • Dapeng Zhang
  • Xiaoyu Wang
  • Juan Chen
Original Paper

Abstract

Dehydrins (DHNs), which are stress-related proteins, are important for plant survival under various abiotic and biotic stresses. To elucidate the regulatory mechanisms of wheat-derived DHNs under these stresses, we characterized the DHN wzy2 promoter of the wheat cultivar Zhengyin 1 and studied its contribution to stress tolerance. Sequence analysis indicated that the wzy2 gene contains one 109-bp intron inserted in the nucleotide sequence encoding the S-motif and characterized by a GT-AG border. The wzy2 promoter was revealed to contain several potential stress-related cis-acting regulatory elements, including elements responsive to abscisic acid (ABA; ABREs), anoxia (GC motifs), low temperature (LTREs), auxin (TGA elements), methyl jasmonate (MeJA; TGACG motifs), and gibberellin (TATC boxes). Quantitative real-time PCR analysis showed that transcript accumulation occurred in response to low temperature, anoxia, indoleacetic acid, MeJA, ABA, and gibberellin (GA) treatments. Histochemical analysis of GUS expression demonstrated that wzy2 promoter activity could be upregulated by low temperature, anoxia, ABA, and GA treatments. Interestingly, wzy2 promoter element-driven β-glucuronidase expression was first observed in meristemoids rather than calli of wheat seeds subjected to anoxia. Taken together, these results indicate that YSK2-type wzy2 can be induced directly by ABA, low temperature, anoxia, and GA treatments and indirectly by drought, implying that different cis-acting elements interact in stress response cross talk.

Keywords

Triticum aestivum L. Dehydrin Promoter Abscisic acid Cis-acting element Stress response 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant no. 31071349) and Specialized Research Fund for the Doctoral Program of Higher Education (no. 20120204110033).

Supplementary material

10142_2013_354_MOESM1_ESM.doc (43 kb)
ESM 1 (DOC 43 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Weining Zhu
    • 1
  • Linsheng Zhang
    • 1
    Email author
  • Hui Lv
    • 1
  • Hongmei Zhang
    • 1
    • 2
  • Dapeng Zhang
    • 1
  • Xiaoyu Wang
    • 1
  • Juan Chen
    • 1
  1. 1.State Key Laboratory of Crop Stress Biology for Arid Areas/College of Life ScienceNorthwest A&F UniversityYanglingChina
  2. 2.College of Food & BioengineeringHenan University of Science and TechnologyLuoyangChina

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