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Functional & Integrative Genomics

, Volume 14, Issue 1, pp 161–175 | Cite as

Genome-wide identification and expression analysis of the mitogen-activated protein kinase gene family from banana suggest involvement of specific members in different stages of fruit ripening

  • Mehar Hasan Asif
  • Deepika Lakhwani
  • Sumya Pathak
  • Sweta Bhambhani
  • Sumit K. Bag
  • Prabodh Kumar TrivediEmail author
Original Paper

Abstract

Mitogen-activated protein kinases (MAPKs) are important components of the tripartite mitogen-activated protein kinase signaling cascade and play an important role in plant growth and development. Although members of the MAPK gene family have been identified in model plants, little information is available regarding this gene family in fruit crops. In this study, we carried out a computational analysis using the Musa Genome database to identify members of the MAPK gene family in banana, an economically important crop and the most popular fruit worldwide. Our analysis identified 25 members of the MAP kinase (MAPK or MPK) gene family. Phylogenetic analyses of MPKs in Arabidopsis, Oryza, and Populus have classified these MPKs into four subgroups. The presence of conserved domains in the deduced amino acid sequences, phylogeny, and genomic organization strongly support their identity as members of the MPK gene family. Expression analysis during ethylene-induced banana fruit ripening suggests the involvement of several MPKs in the ethylene signal transduction pathway that are necessary for banana fruit ripening. Analysis of the cis-regulatory elements in the promoter regions and the involvement of the identified MPKs in various cellular processes, as analyzed using Pathway Studio, suggest a role for the banana MPK gene family in diverse functions related to growth, development, and the stress response. This report is the first concerning the identification of members of a gene family and the elucidation of their role in various processes using the Musa Genome database.

Keywords

Banana Differential gene expression Fruit ripening Genome-wide analysis MAP kinase 

Notes

Acknowledgments

This work was supported by a research grant from the Council of Scientific and Industrial Research, New Delhi, as Network Project (BSC-107). SP acknowledges Council of Scientific and Industrial Research Govt. of India for Senior Research Fellowships. The authors acknowledge Dr. Shirish A. Ranade, Chief Scientist, CSIR-NBRI for editing the manuscript to refine the language.

Supplementary material

10142_2013_349_MOESM1_ESM.docx (13 kb)
Supplementary Table S1 (DOCX 12 kb)
10142_2013_349_MOESM2_ESM.xls (266 kb)
Supplementary Table S2 (XLS 266 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Mehar Hasan Asif
    • 1
  • Deepika Lakhwani
    • 1
  • Sumya Pathak
    • 1
  • Sweta Bhambhani
    • 1
  • Sumit K. Bag
    • 1
  • Prabodh Kumar Trivedi
    • 1
    Email author
  1. 1.National Botanical Research InstituteCouncil of Scientific and Industrial Research (CSIR-NBRI)LucknowIndia

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