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Functional & Integrative Genomics

, Volume 12, Issue 2, pp 265–275 | Cite as

LOX genes in blast fungus (Magnaporthe grisea) resistance in rice

  • Soma S. Marla
  • V. K. Singh
Original Paper

Abstract

Plant Lipoxygenases (LOX) are known to play major role in plant immunity by providing front-line defense against pathogen-induced injury. To verify this, we isolated a full-length OsLOX3 gene and also 12 OsLOX cDNA clones from Oryza sativa indica (cultivar Pusa Basmati 1). We have examined the role played by LOXs in plant development and during attack by blast pathogen Magnaporthe grisea. Gene expression, promoter region analysis, and biochemical and protein structure analysis of isolated OsLOX3 revealed significant homology with LOX super family. Protein sequence comparison of OsLOXs revealed high levels of homology when compared with japonica rice (up to100%) and Arabidopsis (up to 64%). Isolated LOX3 gene and 12 OsLOX cDNAs contained the catalytic LOX domains much required for oxygen binding and synthesis of oxylipins. Amino acid composition, protein secondary structure, and promoter region analysis (with abundance of motifs CGTCA and TGACG) support the role of OsLOX3 gene in providing resistance to diseases in rice plants. OsLOX3 gene expression analysis of root, shoot, flag leaf, and developing and mature seed revealed organ specific patterns during rice plant development and gave evidence to association between tissue location and physiological roles played by individual OsLOXs. Increased defense activity of oxylipins was observed as demonstrated by PCR amplification of OsLOX3 gene and upon inoculation with virulent strains of M. grisea and ectopic application of methyl jasmonate in the injured leaf tissue in adult rice plants.

Keywords

Lipoxygenase Rice Blast disease Defense response Methyl jasmonate Expression 

Notes

Acknowledgments

The authors are grateful to Bioinformatics Information Network under Department of Biotechnology, Government of India for grant provided to support experimental costs and fellowship to VKS. Also, the authors are thankful to B. Murugan and A. Dev for help in disease inoculation experiments in the green house and to Department of Molecular Biology and Genetic engineering, G.B. Pant University of Agriculture and Technology, Pantngar, India for providing all laboratory and green house facilities.

Supplementary material

10142_2012_268_MOESM1_ESM.doc (538 kb)
ESM 1 (DOC 537 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Molecular Biology and Genetic EngineeringG.B. Pant University of Agriculture and TechnologyPantnagarIndia
  2. 2.National Genomic Resources and Bioinformatics, National Bureau of Plant Genetic ResourcesICARNew DelhiIndia
  3. 3.Bioinformatics CentreBanaras Hindu UniversityVaranasiIndia

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