HvNax3—a locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum)
- 590 Downloads
Previous work identified the wild barley (Hordeum vulgare ssp. spontaneum) accession CPI-71284-48 as being capable of limiting sodium (Na+) accumulation in the shoots under saline hydroponic growth conditions. Quantitative trait locus (QTL) analysis using a cross between CPI-71284-48 and a selection of the cultivated barley (H. vulgare ssp. vulgare) cultivar Barque (Barque-73, a moderate Na+ excluder) attributed the control of the Na+ exclusion trait from CPI-71284-48 to a single locus on the short arm of chromosome 7H, which was named HvNax3. The locus reduced shoot Na+ accumulation by 10–25% in plants grown in 150 mM NaCl. Markers generated using colinearity with rice and Brachypodium, together with the analysis of introgression lines and F2 and F3 families, enabled HvNax3 to be mapped to a 1.3-cM interval. Genes from the corresponding rice and Brachypodium intervals encode 16 different classes of proteins and include several plausible candidates for HvNax3. The potential of HvNax3 to provide a useful trait contributing to salinity tolerance in cultivated barley is discussed.
KeywordsSodium transport Barley H. vulgare ssp. spontaneum Salinity tolerance Pyrophosphatase
We thank Jason Eglinton, Stewart Coventry, and Julie Hayes for helpful discussions, Nilmini Dharmathilake and Anita Lapina for technical assistance, Jason Eglinton and Stewart Coventry for sharing unpublished data, the MPB-CRC for genetic resources, and codirectors of the Brachypodium Genome Sequencing Project for permission to use unpublished genomic sequence. This project was supported by a GRDC grant UA00090 to MT, a DBT grant (India) to NKG, research grant of the Institute of International Education (USA) to MNB, and by ARC, GRDC, and South Australian Government funding to ACPFG.
- Eglinton JK, Jefferies SP, Ceccarelli S, Grando S, McDonald G, Barr AR (2000) A comparative assessment of osmotic adjustment and oxidative stress responses in Hordeum vulgare ssp. spontaneum. In: Proceedings of the 8th International Barley Genetics Symposium, Adelaide, pp 267–269Google Scholar
- Eglinton JK, Coventry SJ, Mather DE, Kretschmer J, McMichael GL, Chalmers KJ (2004) Advanced backcross QTL analysis in barley. In: Proceedings of the 9th International Barley Genetics Symposium, Brno, Czech Republic, p 65, http://www.ibgs.cz/
- Ellis RP, Forster BP, Gordon DC, Handley LL, Keith RP, Lawrence P, Meyer R, Powell W, Robinson D, Scrimgeour CM, Young G, Thomas WTB (2002) Phenotype/genotype association for yield and salt tolerance in an barley mapping population segregating for two dwarfing genes. J Exp Bot 53:1163–1176CrossRefPubMedGoogle Scholar
- Forster BP, Miller TE, Law CN (1988) Salt tolerance of two wheat—Agropyron junceum disomic addition lines. Genome 30:559–564Google Scholar
- Guo S, Yin H, Zhang X, Zhao F, Li P, Chen S, Zhao Y, Zhang H (2006) Molecular cloning and characterization of a vacuolar H+-pyrophosphatase gene, SsVP, from the halophyte Suaeda salsa and its overexpression increases salt and drought tolerance of Arabidopsis. Plant Mol Biol 60:41–50CrossRefPubMedGoogle Scholar
- Jensen JK, Sørensen SO, Harholt J, Geshi N, Sakuragi Y, Møller I, Zandleven J, Bernal AJ, Jensen NB, Sørensen C, Pauly M, Beldman G, Willats WGT, Scheller HV (2008) Identification of a xylogalacturonan xylosyltransferase involved in pectin biosynthesis in Arabidopsis. Plant Cell 20:1289–1302CrossRefPubMedGoogle Scholar
- Omielan JA, Epstein E, Dvořák J (1991) Salt tolerance and ionic relations of wheat as affected by individual chromosomes of salt-tolerant Lophopyrum elongatum. Genome 34:961–974Google Scholar
- Quarrie SA, Steed A, Calestani C, Semikhodskii A, Lebreton C, Chinoy C, Steele N, Pljevjakusić W, Weyen J, Schondelmaier J, Habash DZ, Farmer P, Saker L, Clarkson DT, Abugalieva A, Yessimbekova M, Turuspekov Y, Abugalieva S, Tuberosa R, Sanguineti MC, Hollington PA, Aragués R, Royo A, Dodig D (2005) A high-density genetic map of hexaploid wheat (Triticum aestivum L.) from the cross Chinese Spring × SQ1 and its use to compare QTLs for grain yield across a range of environments. Theor Appl Genet 110:865–880CrossRefPubMedGoogle Scholar
- Shavrukov Y, Bowne J, Langridge P, Tester M (2006) Screening for sodium exclusion in wheat and barley. In: Proceedings of the 13th Australian Society of Agronomy Conference, Perth, http://www.regional.org.au/au/asa/2006/concurrent/environment/4581_shavrukoky.htm. Accessed 10 Sep 2006