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Membrane transporters and carbon metabolism implicated in chloride homeostasis differentiate salt stress responses in tolerant and sensitive Citrus rootstocks

  • Javier Brumós
  • José M. Colmenero-Flores
  • Ana Conesa
  • Pedro Izquierdo
  • Guadalupe Sánchez
  • Domingo J. Iglesias
  • María F. López-Climent
  • Aurelio Gómez-Cadenas
  • Manuel Talón
Original Paper

Abstract

Salinity tolerance in Citrus is strongly related to leaf chloride accumulation. Both chloride homeostasis and specific genetic responses to Cl toxicity are issues scarcely investigated in plants. To discriminate the transcriptomic network related to Cl toxicity and salinity tolerance, we have used two Cl salt treatments (NaCl and KCl) to perform a comparative microarray approach on two Citrus genotypes, the salt-sensitive Carrizo citrange, a poor Cl excluder, and the tolerant Cleopatra mandarin, an efficient Cl excluder. The data indicated that Cl toxicity, rather than Na+ toxicity and/or the concomitant osmotic perturbation, is the primary factor involved in the molecular responses of citrus plant leaves to salinity. A number of uncharacterized membrane transporter genes, like NRT1-2, were differentially regulated in the tolerant and the sensitive genotypes, suggesting its potential implication in Cl homeostasis. Analyses of enriched functional categories showed that the tolerant rootstock induced wider stress responses in gene expression while repressing central metabolic processes such as photosynthesis and carbon utilization. These features were in agreement with phenotypic changes in the patterns of photosynthesis, transpiration, and stomatal conductance and support the concept that regulation of transpiration and its associated metabolic adjustments configure an adaptive response to salinity that reduces Cl accumulation in the tolerant genotype.

Keywords

Chloride transport Salt stress Citrus rootstock Genomics Carbon metabolism 

Notes

Acknowledgments

Work at Centro de Genómica was supported by INCO UE project 015453 and Ministerio de Educación y Ciencia-FEDER grant AGL2007-65437-C04-01/AGR. Help and expertise of A. Almenar, A. Boix, A. López, E Blázquez, I. López, I. Sanchís, and M. Sancho are gratefully acknowledged.

Supplementary material

10142_2008_107_MOESM1_ESM.xls (478 kb)
Supplemental Tables (XLS 478 kb)
10142_2008_107_MOESM2_ESM.ppt (490 kb)
Supplemental Figures (PPT 490 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Javier Brumós
    • 1
  • José M. Colmenero-Flores
    • 1
    • 4
  • Ana Conesa
    • 3
  • Pedro Izquierdo
    • 1
  • Guadalupe Sánchez
    • 1
  • Domingo J. Iglesias
    • 1
  • María F. López-Climent
    • 2
  • Aurelio Gómez-Cadenas
    • 2
  • Manuel Talón
    • 1
  1. 1.Instituto Valenciano de Investigaciones AgrariasCentro de GenómicaMoncadaSpain
  2. 2.Departament de Ciències AgràriesUniversitat Jaume I. Campus de Riu Sec (E.S.T.C.E.)Castelló de la PlanaSpain
  3. 3.Department of BioinformaticsCentro de Investigación Príncipe FelipeValenciaSpain
  4. 4.Instituto de Recursos Naturales y AgrobiologíaConsejo Superior de Investigaciones CientíficasSevillaSpain

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