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Functional & Integrative Genomics

, Volume 9, Issue 2, pp 231–242 | Cite as

Stress-related genes define essential steps in the response of maize seedlings to smoke-water

  • Vilmos Soós
  • Endre Sebestyén
  • Angéla Juhász
  • János Pintér
  • Marnie E. Light
  • Johannes Van Staden
  • Ervin Balázs
Original Paper

Abstract

Smoke from burning vegetation is widely recognised as a germination cue for seed germination and recent reports suggest that smoke treatments can improve seedling vigour also. We investigated the effect of smoke-water on seedling vigour and changes of the global transcriptome in the early post-germination phase in maize. Application of smoke-water improved the germination characteristics and seedling vigour. The transcriptional response of embryos and emerging radicles 24 and 48 h after the onset of smoke treatment was investigated. The microarray study revealed a number of smoke-responsive genes amongst which stress- and abscisic acid (ABA)-related genes were over-represented. The global promoter analysis of the smoke-responsive genes revealed a tight correlation with the results obtained from Gene Ontology annotations. This concerted over-expression shows that smoke treatment induces stress and ABA-related responses in the early post-germination phase which leads to better adaptation to environmental stress factors occurring during germination, eventually resulting in greater seedling vigour.

Keywords

Gene expression Germination Maize Smoke Zea may

Notes

Acknowledgments

This work was supported by the Generation Challenge Program (GCP), the Hungarian–South African Intergovernmental S&T Cooperation Programme, the Hungarian Scientific Research Fund (OTKA—F16066), the Baross Gábor Projekt and the National Research Foundation, Pretoria, South Africa. Thanks are due to Ferenc Marincs for his kind advices.

Supplementary material

10142_2008_105_MOESM1_ESM.xls (518 kb)
Supplementary Table S1 List of smoke responsive genes (XLS 518 KB)
10142_2008_105_MOESM2_ESM.xls (110 kb)
Supplementary Table S2 Cis-acting elements found in the predicted promoter sequence of smoke responsive genes (XLS 110 KB)
10142_2008_105_MOESM3_ESM.xls (102 kb)
Supplementary Table S3 Frequency and significance of the GO terms appearing in the smoke responsive genes (XLS 102 KB)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Vilmos Soós
    • 1
  • Endre Sebestyén
    • 1
  • Angéla Juhász
    • 1
  • János Pintér
    • 2
  • Marnie E. Light
    • 3
  • Johannes Van Staden
    • 3
  • Ervin Balázs
    • 1
  1. 1.Department of Applied GenomicsAgricultural Research Institute of the Hungarian Academy of Sciences (ARI-HAS)MartonvásárHungary
  2. 2.Department of Maize BreedingAgricultural Research Institute of the Hungarian Academy of SciencesMartonvásárHungary
  3. 3.Research Centre for Plant Growth and Development, School of Biological and Conservation SciencesUniversity of KwaZulu-Natal PietermaritzburgScottsvilleSouth Africa

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