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Functional & Integrative Genomics

, Volume 8, Issue 2, pp 175–180 | Cite as

Differential gene expression of rice two-component signaling elements during reproductive development and regulation by abiotic stress

  • Mukesh Jain
  • Akhilesh K. Tyagi
  • Jitendra P. KhuranaEmail author
Short Communication

Abstract

The two-component signaling elements have been implicated in diverse cellular processes in plants. Earlier, we reported the identification, characterization and expression analysis of type-A response regulators in rice. In this study, we have comprehensively analyzed the expression profile of all the two-component signaling elements identified in rice at various stages of vegetative and reproductive development by employing microarray analysis. Most of the components are expressed in all the developmental stages analyzed. A few of these were found to be specifically expressed during certain stages of seed development, suggesting their role in embryo and endosperm development. In addition, some of these components express differentially under various abiotic stress conditions, indicating their involvement at various levels of hierarchy in abiotic stress signaling.

Keywords

Abiotic stress Expression profiling Oryza sativa (rice) Reproductive development Two-component signaling elements 

Notes

Acknowledgment

The financial support from the Department of Biotechnology, Government of India, and the University Grants Commission, New Delhi, is gratefully acknowledged. M.J. acknowledges the financial support from the Department of Biotechnology, Government of India, under the Innovative Young Biotechnologists Award scheme.

Supplementary material

10142_2007_63_MOESM1_ESM.doc (22 kb)
Table 1 Primer sequences used for real-time PCR analysis. (DOC 23 kb)
10142_2007_63_MOESM2_ESM.xls (34 kb)
Table 2 Average log signal values of two-component signaling elements in various rice tissue/organs and developmental stages. (XLS 34 kb)
10142_2007_63_MOESM3_ESM.ppt (192 kb)
Fig. 1 Expression profiles of TCS element genes present on duplicated chromosomal segments (a) and present in tandem (b). The average log signal values (from three biological replicates) for each gene in all the samples analyzed is presented. (PPT 192 kb)

References

  1. Anderson A, Hudson M, Chen W, Zhu T (2003) Identification of nutrient partitioning genes participating in rice grain filling by singular value decomposition (SVD) of genome expression data. BMC Genomics 4:26PubMedCrossRefGoogle Scholar
  2. Gattolin S, Alandete-Saez M, Elliott K, Gonzalez-Carranza Z, Naomab E, Powell C, Roberts JA (2006) Spatial and temporal expression of the response regulators ARR22 and ARR24 in Arabidopsis thaliana. J Exp Bot 57:4225–4233PubMedCrossRefGoogle Scholar
  3. Grefen C, Harter K (2004) Plant two-component systems: principles, functions, complexity and cross talk. Planta 219:733–742PubMedCrossRefGoogle Scholar
  4. Heyl A, Schmulling T (2003) Cytokinin signal perception and transduction. Curr Opin Plant Biol 6:480–488PubMedCrossRefGoogle Scholar
  5. Higuchi M, Pischke MS, Mahonen AP, Miyawaki K, Hashimoto Y, Seki M, Kobayashi M, Shinozaki K, Kato T, Tabata S, Helariutta Y, Sussman MR, Kakimoto T (2004) In planta functions of the Arabidopsis cytokinin receptor family. Proc Natl Acad Sci U S A 101:8821–8826PubMedCrossRefGoogle Scholar
  6. Hutchison CE, Kieber JJ (2002) Cytokinin signaling in Arabidopsis. Plant Cell Suppl 14:S47–S59Google Scholar
  7. Hutchison CE, Li J, Argueso C, Gonzalez M, Lee E, Lewis MW, Maxwell BB, Perdue TD, Schaller GE, Alonso JM, Ecker JR, Kieber JJ (2006) The Arabidopsis histidine phosphotransfer proteins are redundant positive regulators of cytokinin signaling. Plant Cell 18:3073–3087PubMedCrossRefGoogle Scholar
  8. Hwang I, Chen HC, Sheen J (2002) Two-component signal transduction pathways in Arabidopsis. Plant Physiol 129:500–515PubMedCrossRefGoogle Scholar
  9. International Rice Genome Sequencing Project (2005) The map-based sequence of the rice genome. Nature 436:793–800CrossRefGoogle Scholar
  10. Ito Y, Kurata N (2006) Identification and characterization of cytokinin-signalling gene families in rice. Gene 382:57–65PubMedCrossRefGoogle Scholar
  11. Itoh J, Nonomura K, Ikeda K, Yamaki S, Inukai Y, Yamagishi H, Kitano H, Nagato Y (2005) Rice plant development: From zygote to spikelet. Plant Cell Physiol 46:23–47PubMedCrossRefGoogle Scholar
  12. Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi AK, Khurana JP (2007) F-box proteins in rice. Genome-wide analysis, classification, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress. Plant Physiol 143:1467–1483PubMedCrossRefGoogle Scholar
  13. Jain M, Tyagi AK, Khurana JP (2006) Molecular characterization and differential expression of cytokinin-responsive type-A response regulators in rice (Oryza sativa). BMC Plant Biol 6:1PubMedCrossRefGoogle Scholar
  14. Kakimoto T (2003) Perception and signal transduction of cytokinins. Annu Rev Plant Biol 54:605–627PubMedCrossRefGoogle Scholar
  15. Lohrmann J, Harter K (2002) Plant two-component signaling systems and the role of response regulators. Plant Physiol 128:363–369PubMedCrossRefGoogle Scholar
  16. Mason MG, Li J, Mathews DE, Kieber JJ, Schaller GE (2004) Type-B response regulators display overlapping expression patterns in Arabidopsis. Plant Physiol 135:927–937PubMedCrossRefGoogle Scholar
  17. Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C (2004) Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis. Plant Cell 16:1365–1377PubMedCrossRefGoogle Scholar
  18. Oka A, Sakai H, Iwakoshi S (2002) His–Asp phosphorelay signal transduction in higher plants: Receptors and response regulators for cytokinin signaling in Arabidopsis thaliana. Genes Genet Syst 77:383–391PubMedCrossRefGoogle Scholar
  19. Pareek A, Singh A, Kumar M, Kushwaha HR, Lynn AM, Singla-Pareek SL (2006) Whole-genome analysis of Oryza sativa reveals similar architecture of two-component signaling machinery with Arabidopsis. Plant Physiol 142:380–397PubMedCrossRefGoogle Scholar
  20. Riefler M, Novak O, Strnad M, Schmulling T (2006) Arabidopsis cytokinin receptor mutants reveal functions in shoot growth, leaf senescence, seed size, germination, root development, and cytokinin metabolism. Plant Cell 18:40–54PubMedCrossRefGoogle Scholar
  21. Schaller GE, Doi K, Hwang I, Kieber JJ, Khurana JP, Kurata N, Mizuno T, Pareek A, Shiu SH, Wu P, Yip WK (2007) Nomenclature for two-component signaling elements of rice. Plant Physiol 143:555–557PubMedCrossRefGoogle Scholar
  22. Schaller GE, Mathews DE, Gribskov M, Walker JC (2002) Two component signaling elements and histidyl–aspartyl phosphorelays. In: Somerville C, Meyerowitz E (eds) The Arabidopsis Book. American Society of Plant Biologists, Rockville, MD, pp 1–9Google Scholar
  23. Stock AM, Robinson VL, Goudreau PN (2000) Two-component signal transduction. Annu Rev Biochem 69:183–215PubMedCrossRefGoogle Scholar
  24. Tajima Y, Imamura A, Kiba T, Amano Y, Yamashino T, Mizuno T (2004) Comparative studies on the type-B response regulators revealing their distinctive properties in the His-to-Asp phosphorelay signal transduction of Arabidopsis thaliana. Plant Cell Physiol 45:28–39PubMedCrossRefGoogle Scholar
  25. Thorne JH (1985) Phloem unloading of C and N assimilates in developing seeds. Annu Rev Plant Physiol 36:317–343CrossRefGoogle Scholar
  26. To JP, Haberer G, Ferreira FJ, Deruere J, Mason MG, Schaller GE, Alonso JM, Ecker JR, Kieber JJ (2004) Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling. Plant Cell 2004 16:658–671CrossRefGoogle Scholar
  27. Urao T, Yakubov B, Satoh R, Yamaguchi-Shinozaki K, Seki M, Hiryama T, Shinozaki K (1999) A transmembrane hybrid-type histidine kinase in Arabidopsis functions as an osmosensor. Plant Cell 11:1743–1754PubMedCrossRefGoogle Scholar
  28. Urao T, Yakubov B, Yamaguchi-Shinozaki K, Shinozaki K (1998) Stress-responsive expression of genes for two-component response regulator-like proteins in Arabidopsis thaliana. FEBS Lett 427:175–178PubMedCrossRefGoogle Scholar
  29. Weijers D, Benkova E, Jager KE, Schlereth A, Hamann T, Kientz M, Wilmoth JC, Reed JW, Jurgens G (2005) Developmental specificity of auxin response by pairs of ARF and Aux/IAA transcriptional regulators. EMBO J 24:1874–1885PubMedCrossRefGoogle Scholar
  30. West AH, Stock AM (2001) Histidine kinases and response regulator proteins in two-component signaling systems. Trends Biochem Sci 26:369–376PubMedCrossRefGoogle Scholar
  31. Whitelaw C, Paul W, Jenkins E, Taylor V, Roberts J (1999) An mRNA encoding a response regulator protein from Brassica napus is up-regulated during pod development. J Exp Bot 50:335–341CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Mukesh Jain
    • 1
  • Akhilesh K. Tyagi
    • 1
  • Jitendra P. Khurana
    • 1
    Email author
  1. 1.Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular BiologyUniversity of Delhi South CampusNew DelhiIndia

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