Functional & Integrative Genomics

, Volume 8, Issue 2, pp 175–180 | Cite as

Differential gene expression of rice two-component signaling elements during reproductive development and regulation by abiotic stress

  • Mukesh Jain
  • Akhilesh K. Tyagi
  • Jitendra P. KhuranaEmail author
Short Communication


The two-component signaling elements have been implicated in diverse cellular processes in plants. Earlier, we reported the identification, characterization and expression analysis of type-A response regulators in rice. In this study, we have comprehensively analyzed the expression profile of all the two-component signaling elements identified in rice at various stages of vegetative and reproductive development by employing microarray analysis. Most of the components are expressed in all the developmental stages analyzed. A few of these were found to be specifically expressed during certain stages of seed development, suggesting their role in embryo and endosperm development. In addition, some of these components express differentially under various abiotic stress conditions, indicating their involvement at various levels of hierarchy in abiotic stress signaling.


Abiotic stress Expression profiling Oryza sativa (rice) Reproductive development Two-component signaling elements 



The financial support from the Department of Biotechnology, Government of India, and the University Grants Commission, New Delhi, is gratefully acknowledged. M.J. acknowledges the financial support from the Department of Biotechnology, Government of India, under the Innovative Young Biotechnologists Award scheme.

Supplementary material

10142_2007_63_MOESM1_ESM.doc (22 kb)
Table 1 Primer sequences used for real-time PCR analysis. (DOC 23 kb)
10142_2007_63_MOESM2_ESM.xls (34 kb)
Table 2 Average log signal values of two-component signaling elements in various rice tissue/organs and developmental stages. (XLS 34 kb)
10142_2007_63_MOESM3_ESM.ppt (192 kb)
Fig. 1 Expression profiles of TCS element genes present on duplicated chromosomal segments (a) and present in tandem (b). The average log signal values (from three biological replicates) for each gene in all the samples analyzed is presented. (PPT 192 kb)


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Mukesh Jain
    • 1
  • Akhilesh K. Tyagi
    • 1
  • Jitendra P. Khurana
    • 1
    Email author
  1. 1.Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular BiologyUniversity of Delhi South CampusNew DelhiIndia

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