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Functional & Integrative Genomics

, Volume 5, Issue 4, pp 260–263 | Cite as

Physical molecular maps of wheat chromosomes

  • Aakash Goyal
  • Rajib Bandopadhyay
  • Pierre Sourdille
  • Takashi R. Endo
  • Harindra S. Balyan
  • Pushpendra K. GuptaEmail author
Short Communication

Abstract

In bread wheat, a set of 527 simple sequence repeats (SSRs) were tried on 164 deletion lines, leading to a successful mapping of 270 SSRs on 313 loci covering all 21 chromosomes. A maximum of 119 loci (38%) were located on B subgenome, and a minimum of 90 loci (29%) mapped on D subgenome. Similarly, homoeologous group 7 carried a maximum of 61 loci (19%), and group 4 carried a minimum of 22 loci (7%). Of the cited 270 SSRs, 39 had multiple loci, but only eight of these detected homoeologous loci. Linear order of loci in physical maps largely corresponded with those in the genetic maps. Apparently, distances between each of only 26 pairs of loci significantly differed from the corresponding distances on genetic maps. Some loci, which were genetically mapped close to the centromere, were physically located distally, while other loci that were mapped distally in the genetic maps were located in the proximal bins in the physical maps. This suggested that although the linear order of the loci was largely conserved, variation does exist between genetic and physical distances.

Keywords

Simple sequence repeats (SSRs) Genetic maps Deletion lines Physical maps 

Notes

Acknowledgements

Thanks are due to Dr. P. Stephenson for providing aliquots of 53 psp SSR primers, to Dr. B. S. Gill for providing the Nulli–Tetrasomic and Ditelosomic lines and to Dr. R. Appels for useful suggestions on the original manuscript. Financial support for this study was provided by the Department of Atomic Energy-Board of Research in Nuclear Science (DAE-BRNS), Government of India, Mumbai, India.

Supplementary material

Fig. 1a-u Physical map of bread wheat and its comparison with available genetic maps

s10142_2005_146_ESM_supp.pdf (1.2 mb)
(PDF 1.2 MB)

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Aakash Goyal
    • 1
  • Rajib Bandopadhyay
    • 1
  • Pierre Sourdille
    • 2
  • Takashi R. Endo
    • 3
  • Harindra S. Balyan
    • 1
  • Pushpendra K. Gupta
    • 1
    Email author
  1. 1.Molecular Biology Laboratory, Department of Genetics & Plant BreedingCh. Charan Singh UniversityMeerut, Uttar PradeshIndia
  2. 2.UMR INRA–UBP Amélioration et Santé des PlantesClermont-FerrandFrance
  3. 3.Graduate School of AgricultureKyoto UniversityKyotoJapan

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