Functional & Integrative Genomics

, Volume 5, Issue 4, pp 201–207 | Cite as

Gene expression profiling of potato responses to cold, heat, and salt stress

  • Willem Albert Rensink
  • Stacey Iobst
  • Amy Hart
  • Svetlana Stegalkina
  • Jia Liu
  • C. Robin Buell
Original Paper

Abstract

In order to identify genes involved in abiotic stress responses in potato, seedlings were grown under controlled conditions and subjected to cold (4°C), heat (35°C), or salt (100 mM NaCl) stress for up to 27 h. Using an ∼12,000 clone potato cDNA microarray, expression profiles were captured at three time points following initiation of the stress (3, 9, and 27 h) from two different tissues, roots and leaves. A total of 3,314 clones could be identified as significantly up- or down-regulated in response to at least one stress condition. The genes represented by these clones encode transcription factors, signal transduction factors, and heat-shock proteins which have been associated with abiotic stress responses in Arabidopsis and rice, suggesting similar response pathways function in potato. These stress-regulated clones could be separated into either stress-specific or shared-response clones, suggesting the existence of general response pathways as well as more stress-specific pathways. In addition, we identified expression profiles which are indicative for the type of stress applied to the plants.

Keywords

Potato Gene expression profiling Abiotic stress 

Supplementary material

s10142_2005_141_ESM_supp.pdf (274 kb)
(PDF 275 KB)

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Willem Albert Rensink
    • 1
  • Stacey Iobst
    • 1
  • Amy Hart
    • 1
  • Svetlana Stegalkina
    • 1
  • Jia Liu
    • 1
  • C. Robin Buell
    • 1
  1. 1.The Institute for Genomic ResearchRockvilleUSA

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