Functional & Integrative Genomics

, Volume 5, Issue 4, pp 201–207 | Cite as

Gene expression profiling of potato responses to cold, heat, and salt stress

  • Willem Albert Rensink
  • Stacey Iobst
  • Amy Hart
  • Svetlana Stegalkina
  • Jia Liu
  • C. Robin Buell
Original Paper


In order to identify genes involved in abiotic stress responses in potato, seedlings were grown under controlled conditions and subjected to cold (4°C), heat (35°C), or salt (100 mM NaCl) stress for up to 27 h. Using an ∼12,000 clone potato cDNA microarray, expression profiles were captured at three time points following initiation of the stress (3, 9, and 27 h) from two different tissues, roots and leaves. A total of 3,314 clones could be identified as significantly up- or down-regulated in response to at least one stress condition. The genes represented by these clones encode transcription factors, signal transduction factors, and heat-shock proteins which have been associated with abiotic stress responses in Arabidopsis and rice, suggesting similar response pathways function in potato. These stress-regulated clones could be separated into either stress-specific or shared-response clones, suggesting the existence of general response pathways as well as more stress-specific pathways. In addition, we identified expression profiles which are indicative for the type of stress applied to the plants.


Potato Gene expression profiling Abiotic stress 



The work on potato abiotic stress and fabrication of potato cDNA microarrays is funded by the National Science Foundation (DBI-9975866 and DBI-0218166). We thank Renae Malek for critical reading of the manuscript.

Supplementary material

s10142_2005_141_ESM_supp.pdf (274 kb)
(PDF 275 KB)


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Willem Albert Rensink
    • 1
  • Stacey Iobst
    • 1
  • Amy Hart
    • 1
  • Svetlana Stegalkina
    • 1
  • Jia Liu
    • 1
  • C. Robin Buell
    • 1
  1. 1.The Institute for Genomic ResearchRockvilleUSA

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