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Targeted mapping of ESTs linked to the adult plant resistance gene Lr46 in wheat using synteny with rice

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Abstract

The gene Lr46 has provided slow-rusting resistance to leaf rust caused by Puccinia triticina in wheat (Triticum aestivum), which has remained durable for almost 30 years. Using linked markers and wheat deletion stocks, we located Lr46 in the deletion bin 1BL (0.84–0.89) comprising 5% of the 1BL arm. The distal part of chromosome 1BL of wheat is syntenic to chromosome 5L of rice. Wheat expressed sequence tags (ESTs) mapping in the terminal 15% of chromosome 1BL with significant homology to sequences from the terminal region of chromosome 5L of rice were chosen for sequence-tagged site (STS) primer design and were mapped physically and genetically. In addition, sequences from two rice bacterial artificial chromosome clones covering the targeted syntenic region were used to identify additional linked wheat ESTs. Fourteen new markers potentially linked to Lr46 were developed; eight were mapped in a segregating population. Markers flanking (2.2 cM proximal and 2.2 cM distal) and cosegregating with Lr46 were identified. The physical location of Lr46 was narrowed to a submicroscopic region between the breakpoints of deletion lines 1BL-13 [fraction length (FL)=0.89–1] and 1BL-10 (FL=0.89–3). We are now developing a high-resolution mapping population for the positional cloning of Lr46.

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Acknowledgements

We would like to thank CIMMYT for providing all the mapping populations, and Dr. Manilal William for his significant suggestions. This work was supported, in part, by a grant from the Kansas Wheat Commission, by USDA-IFAFS competitive grant 2001-04462 and by the Mid-America International Agricultural Consortium. We thank Kim Howell, Jon Raupp and Duane Wilson for assistance in greenhouse experiments, and Steve Brooks and Deven See for technical advice.

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Authors and Affiliations

  1. Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA

    Maria Mateos-Hernandez, Scot H. Hulbert & Bikram S. Gill

  2. Wheat program, CIMMYT, Apartado Postal 6-641, 06600, Mexico, DF, Mexico

    Ravi P. Singh

  3. Plant Science and Entomology Research Unit, USDA-ARS, 4008 Throckmorton Hall, Kansas State University, Manhattan, KS, 66506, USA

    Robert L. Bowden & Gina Brown-Guedira

  4. Campo Experimental Valle de Mexico, INIFAP, Apdo. Postal 10, Chapingo, 56230, Edo de Mexico, Mexico

    Julio Huerta-Espino

Authors
  1. Maria Mateos-Hernandez
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  2. Ravi P. Singh
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  3. Scot H. Hulbert
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  4. Robert L. Bowden
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  5. Julio Huerta-Espino
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  6. Bikram S. Gill
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  7. Gina Brown-Guedira
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Correspondence to Gina Brown-Guedira.

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Mateos-Hernandez, M., Singh, R.P., Hulbert, S.H. et al. Targeted mapping of ESTs linked to the adult plant resistance gene Lr46 in wheat using synteny with rice. Funct Integr Genomics 6, 122–131 (2006). https://doi.org/10.1007/s10142-005-0017-9

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  • DOI: https://doi.org/10.1007/s10142-005-0017-9

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