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Functional & Integrative Genomics

, Volume 4, Issue 2, pp 84–93 | Cite as

Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison

  • Chengdao LiEmail author
  • Peixiang Ni
  • Michael Francki
  • Adam Hunter
  • Yong Zhang
  • David Schibeci
  • Heng Li
  • Allen Tarr
  • Jun Wang
  • Mehmet Cakir
  • Jun Yu
  • Matthew Bellgard
  • Reg Lance
  • Rudi Appels
Original Paper

Abstract

Pre-harvest sprouting results in significant economic loss for the grain industry around the world. Lack of adequate seed dormancy is the major reason for pre-harvest sprouting in the field under wet weather conditions. Although this trait is governed by multiple genes it is also highly heritable. A major QTL controlling both pre-harvest sprouting and seed dormancy has been identified on the long arm of barley chromosome 5H, and it explains over 70% of the phenotypic variation. Comparative genomics approaches among barley, wheat and rice were used to identify candidate gene(s) controlling seed dormancy and hence one aspect of pre-harvest sprouting. The barley seed dormancy/pre-harvest sprouting QTL was located in a region that showed good synteny with the terminal end of the long arm of rice chromosome 3. The rice DNA sequences were annotated and a gene encoding GA20-oxidase was identified as a candidate gene controlling the seed dormancy/pre-harvest sprouting QTL on 5HL. This chromosomal region also shared synteny with the telomere region of wheat chromosome 4AL, but was located outside of the QTL reported for seed dormancy in wheat. The wheat chromosome 4AL QTL region for seed dormancy was syntenic to both rice chromosome 3 and 11. In both cases, corresponding QTLs for seed dormancy have been mapped in rice.

Keywords

Seed dormancy Pre-harvest sprouting Comparative mapping Candidate gene Rice genome sequences 

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Chengdao Li
    • 1
    Email author
  • Peixiang Ni
    • 2
  • Michael Francki
    • 1
    • 3
  • Adam Hunter
    • 4
  • Yong Zhang
    • 2
  • David Schibeci
    • 4
  • Heng Li
    • 2
  • Allen Tarr
    • 1
  • Jun Wang
    • 2
  • Mehmet Cakir
    • 5
  • Jun Yu
    • 2
  • Matthew Bellgard
    • 4
  • Reg Lance
    • 1
  • Rudi Appels
    • 1
    • 5
  1. 1.Department of AgricultureSouth PerthAustralia
  2. 2.Beijing Institute of GenomicsChinese Academy of SciencesBeijingChina
  3. 3.Value-added Wheat CRC-WesternSouth PerthAustralia
  4. 4.Centre of Bioinformatics and Biological ComputingMurdoch UniversityMurdochAustralia
  5. 5.Molecular Plant Breeding CRC-Western Node, SABCMurdoch UniversityMurdochAustralia

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