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Marine Biotechnology

, Volume 2, Issue 3, pp 267–273 | Cite as

Molecular Analysis of Ribosomal RNA Gene of Red Tide Algae Obtained from the Seto Inland Sea

  • Takashi  Hirashita
  • Kazuhiko  Ichimi
  • Shigeru  Montani
  • Mika  Nomura
  • Shigeyuki  Tajima
Article

Abstract

Eleven clones from five species of the planktonic microalgae, (Chattonella antiqua, Chattonella marina, Heterosigma akashiwo, Alexandrium catenella, and Scrippsiella trochoidea), which were collected from the Seto Inland Sea in Japan and from Thailand, were subjected to nucleotide sequence analysis of the D1/D2 domain of the large subunit (LSU) of their ribosomal RNA genes. After amplification by polymerase chain reaction using degenerated primers, whole-nucleotide sequences for the D1/D2 domains of the LSU rRNA gene of 11 microalgae were analyzed. Phylogenic tree analysis using these nucleotide sequences showed each species located in a cluster corresponding to its morphological classification. The nucleotide sequence data for Chattonella spp. suggest that multiple clones of both Chattonella antiqua and Chattonella marina are present in the Seto Inland Sea and that red tide blooms of Chattonella spp. in different years may have contained different clones.

Key words: Red tide, rRNA, Chattonella, Heterosigma, Alexandrium, Scrippsiella 

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Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • Takashi  Hirashita
  • Kazuhiko  Ichimi
    • 2
  • Shigeru  Montani
    • 1
  • Mika  Nomura
    • 1
  • Shigeyuki  Tajima
    • 1
  1. 1.Department of Life Sciences, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0795, JapanJP
  2. 2.Tohoku National Fisheries Research Institute, Shinhama 3-27, Shiogama 5, Miyagi, 985-0001, JapanJP

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