Marine Biotechnology

, Volume 2, Issue 3, pp 248–253 | Cite as

Interspecific Utility of Microsatellites in Fish: A Case Study of (CT)n and (GT)n Markers in the Shanny Lipophrys pholis (Pisces: Blenniidae) and Their Use in Other Blennioidei

  • T.  Guillemaud
  • F.  Almada
  • R.  Serrão Santos
  • M.L.  Cancela
Article

Abstract

We report the development of new microsatellite markers that can be used for population analyses in the shanny Lipophrys pholis. The procedure involved the construction of a microsatellite-enriched genomic bank. Five (GT)n and (CT)n microsatellites have been characterized, four of which are polymorphic. The analysis of one population allowed us to verify their usefulness as markers in population studies. Moreover, interspecific amplifications have been performed using primers defined in other species to amplify Lipophrys pholis, or using the primers defined in Lipophrys pholis to amplify other species. We use these results to discuss the hypothesis that microsatellites are highly conserved in fish.

Key words: Cross-specific amplification, Blenniidae, dinucleotide repeats 

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Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • T.  Guillemaud
    • 1
  • F.  Almada
    • 1
  • R.  Serrão Santos
    • 3
  • M.L.  Cancela
    • 1
  1. 1.Universidade do Algarve, CCMar/UCTRA, P-8000 Faro, PortugalPT
  2. 2.Instituto Superior de Psicologia Aplicada, Unidade de Investigação em Eco-Etologia, Rua Jardim do Tabaco, 44, P-1100 Lisboa, PortugalPT
  3. 3.Universidade dos Açores, Departamento de Oceanografia e Pescas, P-9900 Horta (Açores), PortugalPT

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