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Marine Biotechnology

, Volume 3, Issue 2, pp 91–95 | Cite as

Isolation and Characterization of Four Microsatellite Loci in the Tidewater Goby (Eucyclogobius newberryi)

  • Holly  Mendonca
  • Jerry  Smith
  • Chris  Brinegar

Abstract:

The first microsatellite loci for the endangered tidewater goby Eucyclogobius newberryi are described. A partial tidewater goby genomic library was constructed in pUC19 plasmid. Microsatellite-containing clones were isolated, sequenced, and amplified. Out of 12 positive clones sequenced, 3 were polymorphic and proved useful in a preliminary genetic screen of 3 well-separated populations (50 individuals each) along the central California coast. The number of alleles per locus ranged from 2 to 3. This initial study suggests a population bottleneck had occurred in one of the populations.

Key words:Eucyclogobius newberryi, tidewater goby, microsatellites, population bottleneck. 

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Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Holly  Mendonca
    • 1
  • Jerry  Smith
    • 1
  • Chris  Brinegar
    • 1
  1. 1.Conservation Genetics Laboratory, Department of Biological Sciences, San Jose State University, San Jose, CA 95192, U.S.A.US

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