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Comparative Transcriptome Analysis Reveals Differentially Expressed Genes and Signaling Pathways Between Male and Female Red-Tail Catfish (Mystus wyckioides)

  • Jun-Jie Wu
  • Yu-Lin Zhou
  • Zhong-Wei WangEmail author
  • Guang-Hua Li
  • Fang-Peng Jin
  • Li-Li Cui
  • Hai-Tao Gao
  • Xin-Ping Li
  • Li Zhou
  • Jian-Fang Gui
Original Article

Abstract

Sexual dimorphism is widespread in fish species. The red-tail catfish (Mystus wyckioides) is a commercially important catfish in the lower reaches of the Lancang River and the Mekong basin, and it shows a growth advantage in males. Here, RNA-seq was for the first time used to explore the gene expression difference between the sexes in the hypothalamus and pituitary of red-tail catfish, respectively. In the hypothalamus, 5732 and 271 unigenes have significantly higher and lower expressions, respectively, in males compared with females. KEGG analysis showed that 212 DEGs were annotated to 216 signaling pathways, and enrichment analysis suggested different levels of cAMP and glutamatergic synapse signaling between male and female hypothalami and some of the DEGs appear involved in gonad development and growth. In the pituitary, we found only 19 differentially expressed unigenes, which were annotated to 32 signaling pathways, most of which play important roles in gonad development.

Keywords

Transcriptome Hypothalamus Pituitary Growth Gonad development 

Notes

Funding Information

This work was supported by National Key R&D Program of China (2018YFD0901201), the Key Program of Frontier Sciences of the Chinese Academy of Sciences (QYZDY-SSW-SMC025), the Yunnan Academician Workstation (Y73Z04-1-301), the earmarked fund for Modern Agro-industry Technology Research System (NYCYTX-49), and the Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology (2016FBZ01).

Compliance with Ethical Standards

All experiments and animal treatments were approved by the Institute of Hydrobiology Institutional Animal Care and Use Committee (approval ID, keshuizhuan 0829).

Conflict of Interest

The authors declare that they have no competing interests.

Supplementary material

10126_2019_9894_Fig8_ESM.png (488 kb)
Supplementary Fig. 1

Length distribution of the de novo assembled unignes. (PNG 487 kb)

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High resolution image (TIF 2665 kb)
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Supplementary Fig. 2

Stastics of species distribution of Blast hits and annotated unigenes against NR, KOG, GO, KEGG and SWISSPROT database. (A), Stastics of annotated unigenes in hypothalamus. (B), Stastics of annotated unigenes in pituitary. (C), Species distribution of hypothalamic Blast hits against NR database. (D), Species distribution of pituitary Blast hits against NR database. (PNG 2711 kb)

10126_2019_9894_MOESM2_ESM.tif (61 mb)
High resolution image (TIF 62438 kb)
10126_2019_9894_Fig10_ESM.png (212 kb)
Supplementary Fig. 3

Linear regression analysis of expression data generated by RNA-seq and qRT-PCR. X-axis represents log2(mean normalized RPKM), Y-axis represents log2(mean normalized qRT-PCR). (PNG 212 kb)

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High resolution image (TIF 8647 kb)
10126_2019_9894_MOESM4_ESM.docx (17 kb)
Supplementary Table 1 (DOCX 16 kb)
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Supplementary Table 2 (DOCX 14 kb)
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Supplementary Table 3 (DOCX 16 kb)
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Supplementary Table 4 (DOCX 19 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jun-Jie Wu
    • 1
  • Yu-Lin Zhou
    • 2
  • Zhong-Wei Wang
    • 2
    Email author
  • Guang-Hua Li
    • 1
  • Fang-Peng Jin
    • 1
  • Li-Li Cui
    • 1
  • Hai-Tao Gao
    • 1
  • Xin-Ping Li
    • 3
  • Li Zhou
    • 2
  • Jian-Fang Gui
    • 2
  1. 1.Yunnan Institute of Fishery Sciences ResearchKunmingChina
  2. 2.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of SciencesUniversity of the Chinese Academy of SciencesWuhanChina
  3. 3.Xishuangbanna Native Fish Research and Breeding CenterXishuangbannaChina

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