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Phosphate Enrichment Hampers Development of Juvenile Acropora digitifera Coral by Inhibiting Skeleton Formation

  • Mariko Iijima
  • Ko YasumotoEmail author
  • Jun Yasumoto
  • Mina Yasumoto-Hirose
  • Nami Kuniya
  • Ryota Takeuchi
  • Masashi Nozaki
  • Nobuyoshi Nanba
  • Takashi Nakamura
  • Mitsuru Jimbo
  • Shugo Watabe
Original Article

Abstract

Coral reef degradation due to various local stresses, such as nutrient enrichment and terrestrial run-off into coastal waters, is an increasing global concern. Inorganic phosphates have been considered to possibly inhibit skeleton formation in corals. Despite many studies available on the effects of nutrients on corals, a clear consensus on how nutrients exert deteriorative effects on corals has not been established satisfactorily. In this study, we examined the effects of phosphates and nitrates on in vitro aragonite CaCO3 formation by using biogenic polyamines and in vivo aragonite formation in the skeleton of juvenile Acropora digitifera corals. We showed that the phosphates at similar concentrations clearly inhibited both in vitro and in vivo CaCO3 formation. In contrast, nitrates inhibited neither in vitro aragonite CaCO3 formation nor in vivo aragonite formation in juvenile coral skeleton. Furthermore, our findings showed that inhibition of coral skeleton formation was due to absorption of phosphate on the skeleton, which inorganically inhibited normal development of juvenile coral skeleton.

Keywords

Aragonite formation Calcification Coral skeleton Phosphate enrichment Stress 

Notes

Acknowledgments

We thank Dr. Sakai and Ms. Kinjo of the Tropical Biosphere Research Centre at the University of the Ryukyus Sesoko Station for their assistance in obtaining A. digitifera coral larva.

Funding Information

This study was supported in part by grants-in-aids from the Japan Society for the Promotion of Science (KAKENHI Grant Numbers 25850145, 15K07587, 16K00518, and 18K19237) and by the funding from Kitasato University for Young Researchers.

Compliance with Ethical Standards

Conflict of Interests

The authors declare that they have no competing interests.

Supplementary material

10126_2019_9880_MOESM1_ESM.docx (19.2 mb)
ESM 1 (DOCX 19693 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Mariko Iijima
    • 1
  • Ko Yasumoto
    • 1
    Email author
  • Jun Yasumoto
    • 2
  • Mina Yasumoto-Hirose
    • 3
  • Nami Kuniya
    • 1
  • Ryota Takeuchi
    • 1
  • Masashi Nozaki
    • 2
  • Nobuyoshi Nanba
    • 1
  • Takashi Nakamura
    • 4
  • Mitsuru Jimbo
    • 1
  • Shugo Watabe
    • 1
  1. 1.Kitasato University School of Marine BiosciencesSagamiharaJapan
  2. 2.Department of Regional Agricultural Engineering, Faculty of AgricultureUniversity of the RyukyusOkinawaJapan
  3. 3.Tropical Technology PlusOkinawaJapan
  4. 4.Biology Program, Faculty of ScienceUniversity of the RyukyusOkinawaJapan

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