Marine Biotechnology

, Volume 20, Issue 1, pp 75–86 | Cite as

Cloning, Expression, and Biochemical Characterization of Two New Oligoalginate Lyases with Synergistic Degradation Capability

  • Shangyong Li
  • Linna Wang
  • Xuehong Chen
  • Wenwen Zhao
  • Mi Sun
  • Yantao Han
Original Article


Alginate, the most abundant carbohydrate presents in brown macroalgae, has recently gained increasing attention as an alternative biomass for the production of biofuel. Oligoalginate lyases catalyze the degradation of alginate oligomers into monomers, a prerequisite for bioethanol production. In this study, two new oligoalginate lyase genes, oalC6 and oalC17, were cloned from Cellulophaga sp. SY116, and expressed them in Escherichia coli. The deduced oligoalginate lyases, OalC6 and OalC17, belonged to the polysaccharide lyase (PL) family 6 and 17, respectively. Both showed less than 50% amino acid identity with all of the characterized oligoalginate lyases. Moreover, OalC6 and OalC17 could degrade both alginate polymers and oligomers into monomers in an exolytic mode. Substrate specificity studies demonstrated that OalC6 preferred α-L-guluronate (polyG) blocks, while OalC17 preferred poly β-D-mannuronate (polyM) blocks. The combination of OalC6 and OalC17 showed synergistic degradation ability toward both alginate polymers and oligomers. Finally, an efficient process for the production of alginate monomers was established by combining the new-isolated exotype alginate lyases (i.e., OalC6 and OalC17) and the endotype alginate lyase AlySY08. Overall, our work provides new insights for the development of novel biotechnologies for biofuel production from seaweed.


Oligoalginate lyases Synergistic degradation Alginate monomer Cellulophaga sp. SY116 


Authors’ Contributions

SL and YH conceived the study; SL, LW, and WZ performed the experiments; SL, MS, XC, and YH analyzed the data; XC and SL wrote the paper. All the authors reviewed the manuscript.

Funding Information

This work was supported by National Natural Science Foundation of China (No. 81473384 and 81603337).

Compliance with Ethical Standards

Competing Interests

The authors declare that they have no competing interest.

Supplementary material

10126_2017_9788_MOESM1_ESM.doc (2.4 mb)
ESM 1 (DOC 2483 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Pharmacology, College of basic MedicineQingdao UniversityQingdaoChina
  2. 2.Yellow Sea Fisheries Research Institute, Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of AgricultureChinese Academy of Fishery SciencesQingdaoChina

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