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Marine Biotechnology

, Volume 20, Issue 1, pp 10–19 | Cite as

Mapping QTL for Omega-3 Content in Hybrid Saline Tilapia

  • Grace Lin
  • Le Wang
  • Si Te Ngoh
  • Lianghui Ji
  • Laszlo Orbán
  • Gen Hua Yue
Original Article

Abstract

Tilapia is one of most important foodfish species. The low omega-3 to omega-6 fatty acid ratio in freshwater tilapia meat is disadvantageous for human health. Increasing omega-3 content is an important task in breeding to increase the nutritional value of tilapia. However, conventional breeding to increase omega-3 content is difficult and slow. To accelerate the increase of omega-3 through marker-assisted selection (MAS), we conducted QTL mapping for fatty acid contents and profiles in a F2 family of saline tilapia generated by crossing red tilapia and Mozambique tilapia. The total omega-3 content in F2 hybrid tilapia was 2.5 ± 1.0 mg/g, higher than that (2.00 mg/g) in freshwater tilapia. Genotyping by sequencing (GBS) technology was used to discover and genotype SNP markers, and microsatellites were also genotyped. We constructed a linkage map with 784 markers (151 microsatellites and 633 SNPs). The linkage map was 2076.7 cM long and consisted of 22 linkage groups. Significant and suggestive QTL for total lipid content were mapped on six linkage groups (LG3, -4, -6, -8, -13, and -15) and explained 5.8–8.3% of the phenotypic variance. QTL for omega-3 fatty acids were located on four LGs (LG11, -18, -19, and -20) and explained 5.0 to 7.5% of the phenotypic variance. Our data suggest that the total lipid and omega-3 fatty acid content were determined by multiple genes in tilapia. The markers flanking the QTL for omega-3 fatty acids can be used in MAS to accelerate the genetic improvements of these traits in salt-tolerant tilapia.

Keywords

Tilapia Omega-3 Breeding QTL MAS 

Notes

Acknowledgements

Professor Valerie C. L. Lin is deeply appreciated for her support with this project that is part of Grace Lin’s PhD thesis. We thank our colleague Mr. Baoqing Ye for English editing of the manuscript.

Funding Information

This research was supported by the National Research Foundation, Prime Minister’s Office, Singapore, under its Competitive Research Program (CRP Award No. NRF-CRP7-2010-01)

Supplementary material

10126_2017_9783_MOESM1_ESM.docx (9.4 mb)
Supplementary Fig. 1 (DOCX 9604 kb)
10126_2017_9783_MOESM2_ESM.docx (361 kb)
Supplementary Figures 2–4 QTL for omega-3 content (DOCX 361 kb)
10126_2017_9783_MOESM3_ESM.docx (32 kb)
Supplementary Table 1 (DOCX 32 kb)
10126_2017_9783_MOESM4_ESM.xls (202 kb)
Supplementary Table 2 (XLS 201 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Temasek Life Sciences Laboratory, 1 Research LinkNational University of SingaporeSingaporeSingapore
  2. 2.School of Biological SciencesNanyang Technological UniversitySingaporeSingapore
  3. 3.Department of Animal Sciences and Animal Husbandry, Georgikon FacultyUniversity of PannoniaKeszthelyHungary
  4. 4.Centre for Comparative GenomicsMurdoch UniversityMurdochAustralia
  5. 5.Department of Biological SciencesNational University of SingaporeSingaporeSingapore

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