Marine Biotechnology

, Volume 19, Issue 2, pp 157–163 | Cite as

On-site Direct Detection of Astaxanthin from Salmon Fillet Using Raman Spectroscopy

  • Jun-ichi Hikima
  • Masahiro Ando
  • Hiro-o Hamaguchi
  • Masahiro Sakai
  • Masashi Maita
  • Kazunaga Yazawa
  • Haruko Takeyama
  • Takashi AokiEmail author
Original Article


A new technology employing Raman spectroscopy is attracting attention as a powerful biochemical technique for the detection of beneficial and functional food nutrients, such as carotenoids and unsaturated fatty acids. This technique allows for the dynamic characterization of food nutrient substances for the rapid determination of food quality. In this study, we attempt to detect and measure astaxanthin from salmon fillets using this technology. The Raman spectra showed specific bands corresponding to the astaxanthin present in salmon and the value of astaxanthin (Raman band, 1518 cm−1) relative to those of protein/lipid (Raman band, 1446 cm−1) in the spectra increased in a dose-dependent manner. A standard curve was constructed by the standard addition method using astaxanthin as the reference standard for its quantification by Raman spectroscopy. The calculation formula was established using the Raman bands typically observed for astaxanthin (i.e., 1518 cm−1). In addition, we examined salmon fillets of different species (Atlantic salmon, coho salmon, and sockeye salmon) and five fillets obtained from the locations (from the head to tail) of an entire Atlantic salmon. Moreover, the sockeye salmon fillet exhibited the highest astaxanthin concentration (14.2 mg/kg), while coho salmon exhibited an intermediate concentration of 7.0 mg/kg. The Raman-based astaxanthin concentration in the five locations of Atlantic salmon was more strongly detected from the fillet closer to the tail. From the results, a rapid, convenient Raman spectroscopic method was developed for the detection of astaxanthin in salmon fillets.


Raman spectroscopy Astaxanthin Salmon fillet On-site direct detection 



This work was supported by Grant-in-Aid for Challenging Exploratory Research (KAKENHI Grant number 26660178) from Japan Society for the Promotion of Science (JSPS).


  1. Afseth NK, Bloomfield M, World JP, Matousek PA (2014) Novel approach for subsurface through-skin analysis of salmon using spatially offset Raman spectroscopy (SORS). Appl Spectrosc 68:255–262CrossRefPubMedGoogle Scholar
  2. Ando M, Hamaguchi H (2013) Molecular component distribution imaging of living cells by multivariate curve resolution analysis of space-resolved Raman spectra. J Biomed Opt 19:011016CrossRefGoogle Scholar
  3. Bennedsen M, Wang X, Willén R, Wadström T, Andersen LP (1999) Treatment of H. pylori infected mice with antioxidant astaxanthin reduces gastric inflammation, bacterial load and modulates cytokine release by splenocytes. Immunol Lett 70:185–189CrossRefPubMedGoogle Scholar
  4. Chew BP, Park JS, Wong MW, Wong TS (1999) A comparison of the anticancer activities of dietary beta-carotene, canthaxanthin and astaxanthin in mice in vivo. Anticancer Res 19(3A):1849–1853PubMedGoogle Scholar
  5. Ermakov IE, Ermakova MR, Gellermann W (2006) Quantitative detection of astaxanthin and canthaxanthin in Atlantic salmon by resonance Raman spectroscopy. Proc. SPIE 6078: Photonic Therapeutics and Diagnostics II, 607835. doi: 10.1117/12.644783
  6. Fukuhara K, Inokami Y, Tokumura A, Terao J, Suzuki A (1998) Rate constants for quenching singlet oxygen and activities for inhibiting lipid peroxidation of carotenoids and alpha-tocophenol in liposomes. Lipids 33:751–756CrossRefGoogle Scholar
  7. Guerin M, Huntley ME, Olaizola M (2003) Haematococcus astaxanthin: applications for human health and nutrition. Trends Biotechnol 21:210–216CrossRefPubMedGoogle Scholar
  8. Harris DC (2003) Quantitative chemical analysis, 7th edn. W.H. Freeman, New YorkGoogle Scholar
  9. Henmi H, Hara M, Takeuchi M (1990) Resonance Raman and circular-dichroism studies of astaxanthin and/or canthaxanthin in salmon muscle. Nippon Suisan Gakkaishi 56:1825–1828CrossRefGoogle Scholar
  10. Ikeuchi M, Koyama T, Takahashi J, Yazawa K (2007) Effects of astaxanthin in obese mice fed a high-fat diet. Biosci Biotechnol Biochem 71:893–899CrossRefPubMedGoogle Scholar
  11. Jehlicka J, Edwards HGM, Oren A (2014) Raman spectroscopy of microbial pigments. Appl Environ Microbiol 80:3286–3295CrossRefPubMedPubMedCentralGoogle Scholar
  12. Khare A, Moss GP, Weedon BC, Matthews AD (1973) Identification of astaxanthin in Scottish salmon. Comp Biochem Physiol B 45:971–973CrossRefPubMedGoogle Scholar
  13. Kobayashi M (2000) In vivo antioxidant role of astaxanthin under oxidative stress in the green alga Haematococcus pluvialis. Appl Microbiol Biotechnol 54:550–555CrossRefPubMedGoogle Scholar
  14. Kurihara H, Koda H, Asami S, Kiso Y, Tanaka T (2002) Contribution of the antioxidative property of astaxanthin to its protective effect on the promotion of cancer metastasis in mice treated with restraint stress. Life Sci 70:2509–2520CrossRefPubMedGoogle Scholar
  15. Malmsten C, Lignell A (2008) Dietary supplementation with astaxanthin-rich algal meal improves strength endurance—a double blind placebo controlled study on male students. Carotenoid Sci 13:20–22Google Scholar
  16. Miki W (1991) Biological functions and activities of animal carotenoids. Pure Appl Chem 63:141–146CrossRefGoogle Scholar
  17. Misimi E, Mathiassen JR, Erikson U (2007) Computer vision-based sorting of Atlantic salmon (Salmo salar) fillets according to their color level. J Food Sci 72:S030–S035CrossRefPubMedGoogle Scholar
  18. Moe NH (1990) Key factors in marketing farmed salmon. Proc Nutr Soc New Zeal 15:16–22Google Scholar
  19. Motoyama M, Ando M, Sasaki K, Hamaguchi HO (2010) Differentiation of animal fats from different origins: use of polymorphic features detected by Raman spectroscopy. Appl Spectrosc 64:1244–1250CrossRefPubMedGoogle Scholar
  20. Naguib YM (2000) Antioxidant activities of astaxanthin and related carotenoids. J Agric Food Chem 48:1150–1154CrossRefPubMedGoogle Scholar
  21. Naito Y, Uchiyama K, Aoi W, Hasegawa G, Nakamura N, Yoshida N, Maoka T, Takahashi J, Yoshikawa T (2004) Prevention of diabetic nephropathy by treatment with astaxanthin in diabetic db/db mice. Biofactors 20:49–59CrossRefPubMedGoogle Scholar
  22. Nishida Y, Yamashita E, Miki W (2007) Quenching activities of common hydrophilic and lipophilic antioxidants against singlet oxygen using chemiluminescence detection system. Carotenoid Sci 11:16–20Google Scholar
  23. Ohgami K, Shiratori K, Kotake S, Nishida T, Mizuki N, Yazawa K, Ohno S (2003) Effects of astaxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo. Invest Ophthalmol Vis Sci 44:2694–2701CrossRefPubMedGoogle Scholar
  24. Qiu D, Wu YC, Zhu WL, Yin H, Yi LT (2012) Identification of geometrical isomers and comparison of different isomeric samples of astaxanthin. J Food Sci 77:C934–C940CrossRefPubMedGoogle Scholar
  25. Parker SF (1983) Applications of infrared, Raman, and resonance Raman spectroscopy in biochemistry. Plenum Press, New YorkGoogle Scholar
  26. Sawaki K, Yoshigi H, Aoki K, Koikawa N, Azumane A, Kaneko K, Yamaguchi M (2002) Sports performance benefits from taking natural astaxanthin characterized by visual acuity and muscle fatigue improvements in humans. J Clin Ther Med 18:1085–1100Google Scholar
  27. Schiedt K, Leuenberger FJ, Vecchi M (1981) Natural occurrence of enantiomers and meso-astaxanthin 5. Ex wild salmon (Salmo salar and Oncorhynchus). Helv Chim Acta 64:449–457CrossRefGoogle Scholar
  28. Shahidi F, Metusalach J, Brown JA (1998) Carotenoid pigments in seafoods and aquaculture. Crit Rev Food Sci Nutr 38:1–67Google Scholar
  29. Tauler R (1995) Multivariate curve resolution applied to second order data. Chemom Intell Lab Syst 30:133–146CrossRefGoogle Scholar
  30. Tintchev F, Kuhlmann U, Wackerbarth H, Toepfl S, Heinz V, Knorr D, Hildebrandt P (2009) Redox processes in pressurized smoked salmon studied by resonance Raman spectroscopy. Food Chem 112:482–486CrossRefGoogle Scholar
  31. Vandenabeele P, Edwards HGM, Jehlicka J (2014) The role of mobile instrumentation in novel applications of Raman spectroscopy: archaeometry, geosciences, and forensics. Chem Soc Rev 43:2628–2649CrossRefPubMedGoogle Scholar
  32. Wold JP, Marquardt BJ, Dable BK, Robb D, Hatlen B (2004) Rapid quantification of carotenoids and fat in Atlantic Salmon (Salmo salar L.) by Raman spectroscopy and chemometrics. Appl Spectrosc 58:395–403CrossRefPubMedGoogle Scholar
  33. Yang H, Irudayaraj J (2001) Comparison of near-infrared, Fourier transform-infrared and Fourier transform-Raman methods for determining olive pomace oil adulteration in extra virgin oil. J Am Oil Chem Soc 78:889–895CrossRefGoogle Scholar
  34. Yang H, Irudayaraj J, Paradkar MM (2005) Discriminant analysis of edible oils and fats by FTIR, FT-NIR and FT-Raman spectroscopy. Food Chem 93:25–32CrossRefGoogle Scholar
  35. Ytrestøyl T, Coral-Hinostroza G, Hatlen B, Robb DH, Bjerkeng B (2004) Carotenoid and lipid content in muscle of Atlantic salmon, Salmo salar, transferred to seawater as 0+ or 1+ smolts. Comp Biochem Physiol B Biochem Mol Biol 138:29–40CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Jun-ichi Hikima
    • 1
  • Masahiro Ando
    • 2
  • Hiro-o Hamaguchi
    • 2
    • 3
  • Masahiro Sakai
    • 1
  • Masashi Maita
    • 4
  • Kazunaga Yazawa
    • 2
  • Haruko Takeyama
    • 2
    • 5
  • Takashi Aoki
    • 2
    Email author
  1. 1.Department of Biochemistry and Applied Biosciences, Faculty of AgricultureUniversity of MiyazakiMiyazakiJapan
  2. 2.Integrated Institute for Regulatory Science, Research Organization for Nano and Life InnovationWaseda UniversitySbinjuku-kuJapan
  3. 3.Department of Applied Chemistry and Institute of Molecular ScienceNational Chiao Tung UniversityHsinchuTaiwan
  4. 4.Laboratory of Fish Health ManagementTokyo University of Marine Science and TechnologyMinato-kuJapan
  5. 5.Faculty of Science and EngineeringWaseda UniversitySbinjuku-kuJapan

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