Marine Biotechnology

, Volume 17, Issue 5, pp 523–532 | Cite as

Integration of Transcriptomic and Proteomic Approaches Provides a Core Set of Genes for Understanding of Scallop Attachment

  • Yan Miao
  • Lingling Zhang
  • Yan Sun
  • Wenqian Jiao
  • Yangping Li
  • Jin Sun
  • Yangfan Wang
  • Shi Wang
  • Zhenmin BaoEmail author
  • Weizhi LiuEmail author
Original Article


Attachment is an essential physiological process in life histories of many marine organisms. Using a combination of transcriptomic and proteomic approach, scallop byssal proteins (Sbps) and their associated regulatory network genes were investigated for the first time. We built the first scallop foot transcriptome library, and 75 foot-specific genes were identified. Through integration of transcriptomic-proteomic approach, seven unique Sbps were identified. Of them, three showed significant amino acid sequence homology to known proteins. In contrast, the rest did not show significant protein matches, indicating they are possibly novel proteins. Our transcriptomic and proteomic analyses also suggest that post-translational modification may be one of the significant features for Sbps as well. Taken together, our study provides the first multidimensional collection of a core set of genes that may be potentially involved in scallop byssal attachment.


Scallop Adhesion Byssus Transcriptome Proteomics 



This work was supported by National High Technology Research and Development Program of China (2012AA092204), National Natural Science Foundation of China (31472258) and Natural Science Foundation for Distinguished Young Scholars of Shandong Province (JQ201308).

Supplementary material

10126_2015_9635_MOESM1_ESM.doc (35 kb)
Table S1 All Mfp-relevant genes in GenBank; (DOC 35 kb)
10126_2015_9635_MOESM2_ESM.doc (2.1 mb)
Figure S1 Amino acids sequence alignment for the putative Sbp components with their homologs. (DOC 2141 kb)
10126_2015_9635_MOESM3_ESM.xlsx (18 kb)
ESM 1 The significant enriched terms were highlighted in different sheets for biological process, molecular function and cellular component, respectively. (XLSX 17 kb)


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Yan Miao
    • 1
  • Lingling Zhang
    • 1
  • Yan Sun
    • 1
  • Wenqian Jiao
    • 1
  • Yangping Li
    • 1
  • Jin Sun
    • 2
  • Yangfan Wang
    • 1
  • Shi Wang
    • 1
  • Zhenmin Bao
    • 1
    Email author
  • Weizhi Liu
    • 1
    Email author
  1. 1.Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life SciencesOcean University of ChinaQingdaoChina
  2. 2.Department of BiologyHong Kong Baptist University Hong KongChina

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