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Integration of Transcriptomic and Proteomic Approaches Provides a Core Set of Genes for Understanding of Scallop Attachment

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Abstract

Attachment is an essential physiological process in life histories of many marine organisms. Using a combination of transcriptomic and proteomic approach, scallop byssal proteins (Sbps) and their associated regulatory network genes were investigated for the first time. We built the first scallop foot transcriptome library, and 75 foot-specific genes were identified. Through integration of transcriptomic-proteomic approach, seven unique Sbps were identified. Of them, three showed significant amino acid sequence homology to known proteins. In contrast, the rest did not show significant protein matches, indicating they are possibly novel proteins. Our transcriptomic and proteomic analyses also suggest that post-translational modification may be one of the significant features for Sbps as well. Taken together, our study provides the first multidimensional collection of a core set of genes that may be potentially involved in scallop byssal attachment.

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Acknowledgments

This work was supported by National High Technology Research and Development Program of China (2012AA092204), National Natural Science Foundation of China (31472258) and Natural Science Foundation for Distinguished Young Scholars of Shandong Province (JQ201308).

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Authors and Affiliations

  1. Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China

    Yan Miao, Lingling Zhang, Yan Sun, Wenqian Jiao, Yangping Li, Yangfan Wang, Shi Wang, Zhenmin Bao & Weizhi Liu

  2. Department of Biology, Hong Kong Baptist University, Hong Kong, China

    Jin Sun

Authors
  1. Yan Miao
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  2. Lingling Zhang
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  3. Yan Sun
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  4. Wenqian Jiao
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  5. Yangping Li
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  6. Jin Sun
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  7. Yangfan Wang
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  8. Shi Wang
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  9. Zhenmin Bao
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  10. Weizhi Liu
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Corresponding authors

Correspondence to Zhenmin Bao or Weizhi Liu.

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Table S1

All Mfp-relevant genes in GenBank; (DOC 35 kb)

Figure S1

Amino acids sequence alignment for the putative Sbp components with their homologs. (DOC 2141 kb)

ESM 1

The significant enriched terms were highlighted in different sheets for biological process, molecular function and cellular component, respectively. (XLSX 17 kb)

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Miao, Y., Zhang, L., Sun, Y. et al. Integration of Transcriptomic and Proteomic Approaches Provides a Core Set of Genes for Understanding of Scallop Attachment. Mar Biotechnol 17, 523–532 (2015). https://doi.org/10.1007/s10126-015-9635-y

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  • DOI: https://doi.org/10.1007/s10126-015-9635-y

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