Marine Biotechnology

, Volume 17, Issue 2, pp 144–152 | Cite as

Marine Microorganisms as Source of Stereoselective Esterases and Ketoreductases: Kinetic Resolution of a Prostaglandin Intermediate

  • Valerio De Vitis
  • Benedetta Guidi
  • Martina Letizia Contente
  • Tiziana Granato
  • Paola Conti
  • Francesco Molinari
  • Elena Crotti
  • Francesca Mapelli
  • Sara Borin
  • Daniele Daffonchio
  • Diego Romano
Original Article

Abstract

A screening among bacterial strains isolated from water-brine interface of the deep hypersaline anoxic basins (DHABs) of the Eastern Mediterranean was carried out for the biocatalytical resolution of racemic propyl ester of anti-2-oxotricyclo[2.2.1.0]heptan-7-carboxylic acid (R,S)-1, a key intermediate for the synthesis of d-cloprostenol. Bacillus horneckiae 15A gave highly stereoselective reduction of (R,S)-1, whereas Halomonas aquamarina 9B enantioselectively hydrolysed (R,S)-1; in both cases, enantiomerically pure unreacted (R)-1 could be easily recovered and purified at molar conversion below 57–58 %, showing the potential of DHAB extremophile microbiome and marine-derived enzymes in stereoselective biocatalysis.

Keywords

Esterase Ketoreductase Deep-sea hypersaline anoxic basins (DHABs) Marine enzymes Stereoselective Biocatalysis Prostaglandin 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Valerio De Vitis
    • 1
  • Benedetta Guidi
    • 1
  • Martina Letizia Contente
    • 1
  • Tiziana Granato
    • 1
  • Paola Conti
    • 2
  • Francesco Molinari
    • 1
  • Elena Crotti
    • 1
  • Francesca Mapelli
    • 1
  • Sara Borin
    • 1
  • Daniele Daffonchio
    • 1
    • 3
  • Diego Romano
    • 1
  1. 1.Department of Food Environmental and Nutritional Sciences (DEFENS)University of MilanMilanItaly
  2. 2.Department of Pharmaceutical Sciences (DISFARM)University of MilanMilanItaly
  3. 3.BESE DivisionKing Abdullah University of Science and TechnologyThuwalKingdom of Saudi Arabia

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