Marine Biotechnology

, Volume 16, Issue 6, pp 707–715 | Cite as

Quorum-Quenching Activity of the AHL-Lactonase from Bacillus licheniformis DAHB1 Inhibits Vibrio Biofilm Formation In Vitro and Reduces Shrimp Intestinal Colonisation and Mortality

  • G. Vinoj
  • B. VaseeharanEmail author
  • S. Thomas
  • A. J. Spiers
  • S. Shanthi
Original Article


Vibrio parahaemolyticus is a significant cause of gastroenteritis resulting from the consumption of undercooked sea foods and often cause significant infections in shrimp aquaculture. Vibrio virulence is associated with biofilm formation and is regulated by N-acylated homoserine lactone (AHL)-mediated quorum sensing. In an attempt to reduce vibrio colonisation of shrimps and mortality, we screened native intestinal bacilli from Indian white shrimps (Fenneropenaeus indicus) for an isolate which showed biofilm-inhibitory activity (quorum quenching) against the pathogen V. parahaemolyticus DAHP1. The AHL-lactonase (AiiA) expressed by one of these, Bacillus licheniformis DAHB1, was characterised as having a broad-spectrum AHL substrate specificity and intrinsic resistance to the acid conditions of the shrimp intestine. Purified recombinant AiiA inhibited vibrio biofilm development in a cover slip assay and significantly attenuated infection and mortality in shrimps reared in a recirculation aquaculture system. Investigation of intestinal samples also showed that AiiA treatment also reduced vibrio viable counts and biofilm development as determined by confocal laser scanning microscopy (CLSM) imaging. These findings suggest that the B. licheniformis DAHB1 quorum-quenching AiiA might be developed for use as a prophylactic treatment to inhibit or reduce vibrio colonisation and mortality of shrimps in aquaculture.


AHL-lactonase AiiA Bacillus Biofilm Quorum quenching Vibrio parahaemolyticus 



This work was supported by the Indian University Grants Commission (Grant F. no. 36-5/2008 (SR-)). AS was involved in the preparation of the manuscript but not in the experimental work or data analyses.

Supplementary material

10126_2014_9585_Fig5_ESM.gif (54 kb)
Supp. Figure 1

AiiA protein in vitro biofilm inhibition. Shown here are CLSM and light microscopic (40×) images demonstrating the impact of AiiA on biofilm-formation by V. parahaemolyticus DAHP1-GFP after 24 h; control (A & D), with AiiA (C & B). (GIF 54 kb)

10126_2014_9585_MOESM1_ESM.tif (1.4 mb)
High Resolution Image (TIFF 1,387 kb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • G. Vinoj
    • 1
  • B. Vaseeharan
    • 1
    Email author
  • S. Thomas
    • 2
  • A. J. Spiers
    • 3
  • S. Shanthi
    • 1
  1. 1.Crustacean Molecular Biology and Genomics Laboratory, Department of Animal Health and ManagementAlagappa UniversityKaraikudiIndia
  2. 2.Cholera and Environmental Microbiology Laboratory, Department of Molecular MicrobiologyRajiv Gandhi Centre for BiotechnologyTrivandrumIndia
  3. 3.SIMBIOS CentreAbertay UniversityDundeeUnited Kingdom

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