Marine Biotechnology

, Volume 16, Issue 5, pp 522–537 | Cite as

Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality

  • Nadège Richard
  • Ignacio Fernández
  • Tune Wulff
  • Kristin Hamre
  • Leonor Cancela
  • Luis E. C. Conceição
  • Paulo J. Gavaia
Original Article

Abstract

Nutritional factors strongly influence fish larval development and skeletogenesis, and may induce skeletal deformities. Vitamin K (VK) has been largely disregarded in aquaculture nutrition, despite its important roles in bone metabolism, in γ-carboxylation of Gla proteins, and in regulating gene expression through the pregnane X receptor (Pxr). Since the mechanisms mediating VK effects over skeletal development are poorly known, we investigated the effects of VK-supplementation on skeletal development in Senegalese sole larvae, aiming to identify molecular pathways involved. Larvae were fed live preys enriched with graded levels of phylloquinone (PK) (0, 50, and 250 mg kg−1) and survival rate, growth, VK contents, calcium content and incidence of skeletal deformities were determined, revealing an improvement of larval performance and decreasing the incidence of deformities in VK-supplemented groups. Comparative proteome analysis revealed a number of differentially expressed proteins between Control and Diet 250 associated with key biological processes including skin, muscle, and bone development. Expression analysis showed that genes encoding proteins related to the VK cycle (ggcx, vkor), VK nuclear receptor (pxr), and VK-dependent proteins (VKDPs; oc1 and grp), were differentially expressed. This study highlights the potential benefits of increasing dietary VK levels in larval diets, and brings new insights on the mechanisms mediating the positive effects observed on larval performance and skeletal development.

Keywords

Vitamin K Skeletal deformities Proteomics Gene expression Solea senegalensis Flatfish larvae 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Nadège Richard
    • 1
  • Ignacio Fernández
    • 1
  • Tune Wulff
    • 2
  • Kristin Hamre
    • 3
  • Leonor Cancela
    • 1
    • 4
  • Luis E. C. Conceição
    • 1
    • 5
  • Paulo J. Gavaia
    • 1
  1. 1.CCMAR-CIMAR L.A., Centro de Ciências do Mar do AlgarveUniversidade do AlgarveFaroPortugal
  2. 2.Division of Seafood ResearchDTU FoodKgs. LyngbyDenmark
  3. 3.NIFES, National Institute of Nutrition and Seafood ResearchBergenNorway
  4. 4.Department of Biomedical Sciences and MedicineUniversity of AlgarveFaroPortugal
  5. 5.SPAROS Lda, CRIA - Universidade do AlgarveFaroPortugal

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