Marine Biotechnology

, Volume 15, Issue 1, pp 63–72

Genomic Mining for Novel FADH2-Dependent Halogenases in Marine Sponge-Associated Microbial Consortia

  • Kristina Bayer
  • Matthias Scheuermayer
  • Lars Fieseler
  • Ute Hentschel
Original Article

DOI: 10.1007/s10126-012-9455-2

Cite this article as:
Bayer, K., Scheuermayer, M., Fieseler, L. et al. Mar Biotechnol (2013) 15: 63. doi:10.1007/s10126-012-9455-2

Abstract

Many marine sponges (Porifera) are known to contain large amounts of phylogenetically diverse microorganisms. Sponges are also known for their large arsenal of natural products, many of which are halogenated. In this study, 36 different FADH2-dependent halogenase gene fragments were amplified from various Caribbean and Mediterranean sponges using newly designed degenerate PCR primers. Four unique halogenase-positive fosmid clones, all containing the highly conserved amino acid motif “GxGxxG”, were identified in the microbial metagenome of Aplysina aerophoba. Sequence analysis of one halogenase-bearing fosmid revealed notably two open reading frames with high homologies to efflux and multidrug resistance proteins. Single cell genomic analysis allowed for a taxonomic assignment of the halogenase genes to specific symbiotic lineages. Specifically, the halogenase cluster S1 is predicted to be produced by a deltaproteobacterial symbiont and halogenase cluster S2 by a poribacterial sponge symbiont. An additional halogenase gene is possibly produced by an actinobacterial symbiont of marine sponges. The identification of three novel, phylogenetically, and possibly also functionally distinct halogenase gene clusters indicates that the microbial consortia of sponges are a valuable resource for novel enzymes involved in halogenation reactions.

Keywords

FADH2-dependent halogenases Metagenomics Single cell genomics Sponges Porifera Microbial consortia Microbial symbionts 

Supplementary material

10126_2012_9455_MOESM1_ESM.doc (98 kb)
ESM 1(DOC 98 kb)

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Kristina Bayer
    • 1
  • Matthias Scheuermayer
    • 2
  • Lars Fieseler
    • 3
  • Ute Hentschel
    • 1
  1. 1.Julius-von-Sachs Institute for Biological SciencesUniversity of WuerzburgWuerzburgGermany
  2. 2.Center for Infectious DiseasesUniversity of WuerzburgWuerzburgGermany
  3. 3.Institute for Food and Beverage InnovationZurich University of Applied SciencesWädenswilSwitzerland

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