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Marine Biotechnology

, Volume 14, Issue 6, pp 681–691 | Cite as

Diversity of Bacterial Communities Associated with the Indian Ocean Sponge Tsitsikamma favus That Contains the Bioactive Pyrroloiminoquinones, Tsitsikammamine A and B

  • Tara A. Walmsley
  • Gwynneth F. Matcher
  • Fan Zhang
  • Russell T. Hill
  • Michael T. Davies-Coleman
  • Rosemary A. DorringtonEmail author
Original Article

Abstract

Tsitsikamma favus is a latrunculid sponge endemic to the coast of South Africa that produces unique pyrroloiminoquinones known as tsitsikammamines. Wakayin and makaluvamine A are structurally similar to the tsitsikammamines and are the only pyrroloiminoquinones isolated from a source other than Porifera (namely a Fijian ascidian Clavelina sp. and a laboratory culture of the myxomycete Didymium bahiense, respectively). The source of the tsitsikammamines is hypothesised to be microbial, which could provide a means of overcoming the current supply problem. This study focuses on characterising the microbial diversity associated with T. favus. We have used denaturing gradient gel electrophoresis together with clonal and deep sequencing of microbial 16S rRNA gene amplicons to show that specimens of this sponge species contain a distinct and conserved microbial population, which is stable over time and is dominated by a unique Betaproteobacterium species.

Keywords

Tsitsikamma favus Tsitsikammamine Bacterial community 16S rRNA Pyrosequencing 

Notes

Acknowledgements

This research was supported by a grant from the South African National Department of Environmental Affairs as well as funding from the US National Science Foundation Physiological and Structural Systems, Division of Integrative and Organismal Systems (IOS-0919728). TW was funded by Fellowships from the South African National Research Foundation (NRF) and the German Academic Exchange Service (DAAD). GM was supported by a NRF Innovation Postdoctoral Fellowship. FZ was supported by a Fulbright Fellowship. Opinions expressed and conclusions arrived at are those of the authors and are not necessarily to be attributed to any of the above-mentioned donors. The authors wish to thank Dr Toufiek Samaai (Oceans and Coasts, South African Department of Environmental Affairs) and Dr Shirley Parker-Nance for their help in collection and identification of sponge specimens.

Supplementary material

10126_2012_9430_MOESM1_ESM.rtf (11.5 mb)
ESM 1 (RTF 11753 kb)

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Tara A. Walmsley
    • 1
  • Gwynneth F. Matcher
    • 1
  • Fan Zhang
    • 2
  • Russell T. Hill
    • 2
  • Michael T. Davies-Coleman
    • 3
  • Rosemary A. Dorrington
    • 1
    Email author
  1. 1.Department of Biochemistry, Microbiology and BiotechnologyRhodes UniversityGrahamstownSouth Africa
  2. 2.Institute of Marine and Environmental TechnologyUniversity of Maryland Center for Environmental ScienceBaltimoreUSA
  3. 3.Department of ChemistryRhodes UniversityGrahamstownSouth Africa

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