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Marine Biotechnology

, Volume 13, Issue 5, pp 1017–1026 | Cite as

Induced Gynogenesis in Grass Carp (Ctenopharyngodon idellus) Using Irradiated Sperm of Allotetraploid Hybrids

  • Hong Zhang
  • ShaoJun LiuEmail author
  • Chun Zhang
  • Min Tao
  • LiangYue Peng
  • CuiPing You
  • Jun Xiao
  • Yi Zhou
  • GongJian Zhou
  • KaiKun Luo
  • Yun Liu
Original Article

Abstract

Grass carp (Ctenopharyngodon idellus) eggs were activated by UV-irradiated diploid sperm of allotetraploid hybrids derived from red crucian carp (♀) × common carp (♂) and then duplicated by cold shock in 4–6°C water for 10–12 min. Different cold shock initiation times resulted in two types of diploid gynogenetic grass carp: meiotic gynogenetic (meiG) and mitotic gynogenetic (mitG). Over a 5-year period, a total of 17,170 meiG and 1,080 mitG fry were produced and 6,862 meiG and 372 mitG grass carp survived. The gynogenetic fish were confirmed by morphological characteristics, chromosome examination, and microsatellite DNA analysis. The morphological traits of the gynogenetic grass carp were similar to those of wild diploid grass carp. Normal gynogenetic fish were identified as diploid with 48 chromosomes by chromosomal metaphases examination, while nonviable abnormal embryos were detected as haploid with 24 chromosomes. Microsatellite DNA analysis indicated that after one generation of gynogenesis, the genetic purity of meiG and mitG grass carp was significantly increased over that of wild grass carp. In addition, both meiG and mitG grass carp groups were 100% female, and 88% of these showed normal ovary development. Thus, the sex determination mechanism in female grass carp was homogamety. The ability to establish pure all-female groups of meiG and mitG grass carp should be a valuable contribution to both fish genetics and grass carp breeding.

Keywords

Grass carp Gynogenesis Microsatellite Sex determination 

Notes

Acknowledgments

This research was supported by grants from the National Science Fund for Distinguished Young Scholars (no. 30725028), the National Natural Science Foundation of China (no. 30930071), and the State Key Basic Research Project of China (abbreviate: 973 project, grant no. 2007CB109206).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Hong Zhang
    • 1
  • ShaoJun Liu
    • 1
    Email author
  • Chun Zhang
    • 1
  • Min Tao
    • 1
  • LiangYue Peng
    • 1
  • CuiPing You
    • 1
  • Jun Xiao
    • 1
  • Yi Zhou
    • 1
  • GongJian Zhou
    • 1
  • KaiKun Luo
    • 1
  • Yun Liu
    • 1
  1. 1.Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life SciencesHunan Normal UniversityChangshaPeople’s Republic of China

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