The Protective Effect of ENA Actimineral Resource A on CCl4-Induced Liver Injury in Rats
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ENA Actimineral Resource A (ENA-A) is alkaline water that is composed of refined edible cuttlefish bone and two different species of seaweed, Phymatolithon calcareum and Lithothamnion corallioides. In the present study, ENA-A was investigated as an antioxidant to protect against CCl4-induced oxidative stress and hepatotoxicity in rats. Liver injury was induced by either subacute or chronic CCl4 administration, and the rats had free access to tap water mixed with 0% (control group) or 10% (v/v) ENA-A for 5 or 8 weeks. The results of histological examination and measurement of antioxidant activity showed that the reactive oxygen species production, lipid peroxidation, induction of CYP2E1 were decreased and the antioxidant activity, including glutathione and catalase production, was increased in the ENA-A groups as compared with the control group. On 2-DE gel analysis of the proteomes, 13 differentially expressed proteins were obtained in the ENA-A groups as compared with the control group. Antioxidant proteins, including glutathione S-transferase, kelch-like ECH-associated protein 1, and peroxiredoxin 1, were increased with hepatocyte nuclear factor 3-beta and serum albumin precursor, and kininogen precursor decreased more in the ENA-A groups than compared to the control group. In conclusion, our results suggest that ENA-A does indeed have some protective capabilities against CCl4-induced liver injury through its antioxidant function.
KeywordsENA actimineral resource A Antioxidant capability Hepatoxicity Seaweed Carbon tetrachloride
Conflicts of Interest
The authors certify that they have nothing to disclose and no financial interests pertaining to this manuscript.
- Aebi H (1984) Catalase in vitro. In: Packer L (ed) Methods in Enzymology, vol. 105. Academic, San Diego, pp 121–126Google Scholar
- Arakawa Y, Moriyama M, Tanaka N (2003) Liver disease and trace elements. Bioelements in clinical practice (vol. 1 of the series). In: Arakawa Y (ed) Liver disease and bioelements. Academic conference center Kansai/Business center for Academic Societis Japan, Osaka, pp 1–30Google Scholar
- Cesaratto L, Vascotto C, D’Ambrosio, Scaloni A, Baccarani U, Paron I, Damante G, Calligaris S, Quadrifoglo F, Tiribelli C, Tell G (2005) Overoxidation of peroxiredoxins as an immediate and sensitive marker of oxidative stress in HepG2 cells and its application to the redox effects induced by ischemia/reperfusion in human liver. Free Radic Res 39:255–268PubMedCrossRefGoogle Scholar
- Covic A, Kothawala P, Bernal M, Robbins S, Chalian A, Goldsmith D (2009) Systematic review of the evidence underlying the association between mineral metabolism disturbances and risk of all-cause mortality, cardiovascular mortality and cardiovascular events in chronic kidney disease. Nephrol Dial Transplant 24:1506–1523PubMedCrossRefGoogle Scholar