Marine Biotechnology

, Volume 12, Issue 3, pp 273–281 | Cite as

Proteomic Profiling of Liver from Atlantic Salmon (Salmo salar) Fed Genetically Modified Soy Compared to the Near-Isogenic non-GM Line

  • Nini H. Sissener
  • Samuel A. M. Martin
  • Phillip Cash
  • Ernst M. Hevrøy
  • Monica Sanden
  • Gro-Ingunn Hemre
original Article

Abstract

The aim of this study was to investigate potential differences in liver protein expression of Atlantic salmon fed genetically modified (GM) Roundup Ready® soy at a high inclusion level (25% inclusion, constituting 21% of crude protein in the diet) for 7 months or a compositionally similar non-GM diet. The liver was selected as the target organ due to its importance in the general metabolism, and 2D gel electrophoresis used as a screening tool. Samples from 12 individual fish from each diet group were evaluated. Of a total of 781 analysed protein spots, only 36 were significantly different by ANOVA (p < 0.05) in abundance between the diet groups. All these spots had low fold differences (1.2–1.6) and high false discovery rate (q = 0.44), indicating minor differences in liver protein synthesis between fish fed GM and non-GM soy. Additionally, low fold differences were observed. Four protein spots were analyzed by liquid chromatography tandem mass spectrometry and identified using a combination of online searches in NCBI and searches in an inhouse database containing salmonid expressed sequence tags and contigs. Follow-up on these proteins by real-time polymerase chain reaction did not identify differences at the transcriptional level.

Keywords

Atlantic salmon Biotechnology Genetic modification Liver Roundup Ready® soybean 

Supplementary material

10126_2009_9214_MOESM1_ESM.doc (68 kb)
Table 1Protein identifications; matching peptides obtained when searching the NCBI and the EST/contig databases, respectively. When several matches are made to the same amino acid sequence, only the one with the highest ion score is given in the table. O@M oxidation of methionine. (DOC 67 kb)

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Nini H. Sissener
    • 1
  • Samuel A. M. Martin
    • 2
  • Phillip Cash
    • 3
  • Ernst M. Hevrøy
    • 1
  • Monica Sanden
    • 1
  • Gro-Ingunn Hemre
    • 1
  1. 1.National Institute of Seafood and Nutrition Research (NIFES)BergenNorway
  2. 2.School of Biological SciencesUniversity of AberdeenAberdeenUK
  3. 3.Division of Applied Medicine, School of Medical SciencesUniversity of AberdeenAberdeenUK

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