Myxinidin, A Novel Antimicrobial Peptide from the Epidermal Mucus of Hagfish, Myxine glutinosa L.
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Fish epidermal mucus contains innate immune components that provide a first line of defense against various infectious pathogens. This study reports the bioassay-guided fractionation and characterization of a novel antimicrobial peptide, myxinidin, from the acidic epidermal mucus extract of hagfish (Myxine glutinosa L.). Edman sequencing and mass spectrometry revealed that myxinidin consists of 12 amino acids and has a molecular mass of 1,327.68 Da. Myxinidin showed activity against a broad range of bacteria and yeast pathogens at minimum bactericidal concentration (MBC) ranging from 1.0 to 10.0 µg/mL. Screened pathogens, Salmonella enterica serovar Typhimurium C610, Escherichia coli D31, Aeromonas salmonicida A449, Yersinia ruckeri 96-4, and Listonella anguillarum 02-11 were found to be highly sensitive to myxinidin at the MBC of 1.0–2.5 µg/mL; Staphylococcus epidermis C621 and yeast (Candida albicans C627) had an MBC of 10.0 µg/mL. The antimicrobial activity of myxinidin was found to be two to 16 times more active than a potent fish-derived antimicrobial peptide, pleurocidin (NRC-17), against most of the screened pathogens. The microbicidal activity of myxinidin was retained in the presence of sodium chloride (NaCl) at concentrations up to 0.3 M and had no hemolytic activity against mammalian red blood cells. These results suggest that myxinidin may have potential applications in fish and human therapeutics.
KeywordsMucus Antimicrobial peptide Innate immunity Hagfish Myxinidin
The authors would like to thank Mr. Eric MacKinnon (Cape Sable Island, NS) for providing the hagfishes; Ron Melanson for the help with sampling; Cheryl Craft and Dr. Roger Ebanks for assisting in the mucus extraction and antimicrobial assays used in this study; Elden Rowland for the assisting in the mass spectrometric analysis; and Drs. K. Vanya Ewart, Stewart Johnson, and Susan E. Douglas for providing the bacterial cultures. This research was funded by the National Research Council Canada and the Ford Foundation International Fellowship Program.
- Bardack D (1998) Relationships of living and fossil hagfishes. In: Jorgensen JM, Lomholt JP, Weber RE, Malte H (eds) The Biology of Hagfishes. Chapman and Hall, London, U.K., pp 3–14Google Scholar
- Fouz B, Devesa S, Gravningen K, Barja JL, Toranzo AE (1990) Antibacterial action of the mucus of turbot. Bull Eur Assoc Fish Pathol 10:56–59Google Scholar
- Hwang EY, Seo JK, Kim CH, Go HJ, Kim EJ, Chung JK, Ryu HS, Park NG (1999) Purification and characterization of a novel antimicrobial peptide from the skin of the hagfish, Eptatretus burgeri. J Food Sci 4:28–32Google Scholar
- Kennedy J, Baker P, Piper C, Cotter PD, Walsh M, Mooij MJ, Bourke MB, Rea MC, O'Connor PM, Ross RP, Hill C, O'Gara F, Marchesi JR, Dobson ADW (2008) Isolation and analysis of bacteria with antimicrobial activitites from marine sponge Haliclona simulans collected from Irish waters. Mar Biotechnol. doi:10.1007/s10126-008-9154-1 Google Scholar
- Nagashima Y, Kikuchi N, Shimakura K, Shiomi K (2003) Purification and characterization of an antibacterial factor in the skin secretion of rock fish Sebastes schlegeli. Comp Biochem Physiol 136C:63–71Google Scholar
- Raison RL, dos Remedios NJ (1998) The hagfish immune system. In: Jorgensen JM, Lomholt JP, Weber RE, Malte H (eds) The Biology of Hagfishes. U.K.:Chapman and Hall, London, pp 334–344Google Scholar
- Sarmaşik A (2002) Antimicrobial peptides: a potential therapeutic alternative for the treatment of fish diseases. Turk J Biol 26:201–207Google Scholar
- Shinnar AE, Uzzell T, Rao MN, Spooner E, Lane WS, Zasloff M (1996) New family of linear antimicrobial peptides from hagfish intestine contains bromotryptophan as novel amino acid. In: Kaumaya PTP, Hodges RS (eds) Proceedings of the fourteenth American peptide symposium. U.S.A. Mayflower Scientific Ltd., Ohio, pp 189–191Google Scholar
- Subramanian S, MacKinnon SL, Ross NW (2007) A comparative study on innate immune parameters in the epidermal mucus of various fish species. Comp Biochem Physiol 148B:256–263Google Scholar
- Subramanian S, Ross NW, MacKinnon SL (2008) Comparison of antimicrobial activity in the epidermal mucus extracts of fish. Comp Biochem Physiol 150B:85–92Google Scholar
- Yano T (1996) The non-specific immune system: humoral defence. In: Iwama G, Nakanishi T (eds) The fish Immune system: Organism, Pathogen and Environment. Academic press, San Diego, U.S.A, pp 105–157Google Scholar