Marine Biotechnology

, Volume 11, Issue 4, pp 513–519 | Cite as

Profiling the Transcriptome of Gracilaria changii (Rhodophyta) in Response to Light Deprivation

  • Chai-Ling Ho
  • Seddon Teoh
  • Swee-Sen Teo
  • Raha Abdul Rahim
  • Siew-Moi Phang
Original Article

Abstract

Light regulates photosynthesis, growth and reproduction, yield and properties of phycocolloids, and starch contents in seaweeds. Despite its importance as an environmental cue that regulates many developmental, physiological, and biochemical processes, the network of genes involved during light deprivation are obscure. In this study, we profiled the transcriptome of Gracilaria changii at two different irradiance levels using a cDNA microarray containing more than 3,000 cDNA probes. Microarray analysis revealed that 93 and 105 genes were up- and down-regulated more than 3-fold under light deprivation, respectively. However, only 50% of the transcripts have significant matches to the nonredundant peptide sequences in the database. The transcripts that accumulated under light deprivation include vanadium chloroperoxidase, thioredoxin, ferredoxin component, and reduced nicotinamide adenine dinucleotide dehydrogenase. Among the genes that were down-regulated under light deprivation were genes encoding light harvesting protein, light harvesting complex I, phycobilisome 7.8 kDa linker polypeptide, low molecular weight early light-inducible protein, and vanadium bromoperoxidase. Our findings also provided important clues to the functions of many unknown sequences that could not be annotated using sequence comparison.

Keywords

cDNA microarray Irradiance Gracilaria changii Light deprivation Seaweed 

Supplementary material

10126_2008_9166_MOESM1_ESM.doc (134 kb)
Supplementary Table 1Transcripts that were up-regulated at light deprivation. (DOC 133 KB)
10126_2008_9166_MOESM2_ESM.doc (160 kb)
Supplementary Table 2Transcripts that were down-regulated at light deprivation. (DOC 159 KB)

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Chai-Ling Ho
    • 1
  • Seddon Teoh
    • 1
  • Swee-Sen Teo
    • 1
  • Raha Abdul Rahim
    • 1
  • Siew-Moi Phang
    • 2
  1. 1.Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra MalaysiaUPM SerdangMalaysia
  2. 2.Institute of Biological SciencesUniversity of MalayaKuala LumpurMalaysia

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