Marine Biotechnology

, Volume 11, Issue 3, pp 410–418

Isolation and Characterisation of a Δ5-fatty Acid Elongase from the Marine Microalga Pavlova salina

  • Stanley S. Robert
  • James R. Petrie
  • Xue-Rong Zhou
  • Maged P. Mansour
  • Susan I. Blackburn
  • Allan G. Green
  • Surinder P. Singh
  • Peter D. Nichols
Original Article

Abstract

The marine microalga Pavlova salina (Haptophyta, Pavlovophyceae) produces lipids containing approximately 50% n-3 long-chain polyunsaturated fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). A full-length cDNA sequence, designated PsElo5, was isolated from P. salina. Sequence alignment showed that the gene was homologous to corresponding ELO-type elongases from other microalgae. Heterologous expression of PsElo5 in yeast and in higher plants confirmed that it encodes a specific Δ5-elongase activity as predicted and, furthermore, within the n-3 pathway, the elongation activity was confined exclusively to EPA.

Keywords

n-3 LC-PUFA Δ5 elongase Pavlova salina Yeast model Arabidopsis model 

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Stanley S. Robert
    • 1
  • James R. Petrie
    • 2
  • Xue-Rong Zhou
    • 2
  • Maged P. Mansour
    • 1
  • Susan I. Blackburn
    • 1
  • Allan G. Green
    • 3
  • Surinder P. Singh
    • 2
  • Peter D. Nichols
    • 1
  1. 1.Food Futures National Research FlagshipCSIRO Marine and Atmospheric ResearchHobartAustralia
  2. 2.Food Futures National Research FlagshipCSIRO Plant IndustryCanberraAustralia
  3. 3.CSIRO Plant IndustryCanberraAustralia

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