Marine Biotechnology

, Volume 11, Issue 3, pp 317–326 | Cite as

Functional Annotation and Analysis of Expressed Sequence Tags from the Hepatopancreas of Mitten Crab (Eriocheir sinensis)

  • Hui Jiang
  • Yi-Mei Cai
  • Li-Qiao Chen
  • Xiao-Wei Zhang
  • Song-Nian Hu
  • Qun WangEmail author
Original Article


Due to its popularity as a traditional food, intensive harvesting of the mitten crab (Eriocheir sinensis) is common and has lead to an increase in disease incidence, resulting in catastrophic losses to crab aquaculture. The hepatopancreas of E. sinensis is not only an important digestive organ but also an indispensable immune organ. We constructed a nonnormalized cDNA library from the hepatopancreas of E. sinensis and acquired 3,297 high-quality expressed sequence tags representing 1,178 unigenes. More than half of these unigenes were novel genes for this species; the remaining had homologs in public databases, which is of great importance for future functional research. We also investigated the association of these genes with immune processes for insight into one of the main functions of the hepatopancreas besides metabolism. Despite the relatively low sampling scalar of our cDNA library, we were able to demonstrate several important properties of the hepatopancreatic transcriptome and identified numerous genes that were closely associated with immune responses. These results might serve as the basis for an in-depth genomics study of E. sinensis, including transcriptome analysis, physical mapping, and whole genome sequencing.


Eriocheir sinensis Hepatopancreas Expressed sequence tag Invertebrates Innate immunity 



This research was supported by grants from the National Nature Science Foundation of China (no. 30671607), the Key Basic Research Project (06DJ14003) from the Science and Technology Commission of the Shanghai Municipal Government, Zhejiang Key Science and Technology Program (2005C12006-1), and the Specialized Project for the Key Technology Research of Agricultural Restructuring from the Ministry of Agriculture.

Supplementary material

10126_2008_9146_Fig1_ESM.gif (57 kb)
Supplementary file 1

ESTs assigned in contigs; 45% of the contigs yielded by Phrap were formed by two EST sequences and nearly 16% of the contigs were constructed by more than ten ESTs (GIF 57.0 kb)

10126_2008_9146_Fig1_ESM.tif (275 kb)
High resolution image file (TIFF 275 KB)
10126_2008_9146_MOESM3_ESM.doc (54 kb)
Supplementary file 2 The total list of unigenes involved in GO mapping at the second level based on the categories of cellular component, molecular function, and biological process (DOC 53.5 KB)
10126_2008_9146_MOESM4_ESM.doc (76 kb)
Supplementary file 3 Genes expressed at a high level in the library (DOC 76.0 KB)
10126_2008_9146_MOESM5_ESM.doc (132 kb)
Supplementary file 4 The total list of unigenes involved in GO mapping at the third level based on the categories of cellular component, molecular function, and biological process (DOC 132 KB)
10126_2008_9146_MOESM6_ESM.doc (101 kb)
Supplementary file 5 Genes potentially included in stress defense and immune response mechanisms (DOC 101 KB)


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Hui Jiang
    • 1
  • Yi-Mei Cai
    • 2
  • Li-Qiao Chen
    • 1
  • Xiao-Wei Zhang
    • 2
  • Song-Nian Hu
    • 2
  • Qun Wang
    • 1
    Email author
  1. 1.School of Life ScienceEast China Normal UniversityShanghaiChina
  2. 2.Key Laboratory of Genome Sciences and Information, Beijing Institute of GenomicsChinese Academy of SciencesBeijingChina

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