Inhibitory Effect of Deep-sea Water on Differentiation of 3T3-L1 Adipocytes
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Currently, the utilization of deep-sea water (DSW) is receiving much attention due to its high productivity, large quantity, and potential for biological application. The 3T3-L1 cell line is a well-established and commonly used in vitro model to assess adipocyte differentiation. Over the course of several days, confluent 3T3-L1 cells can be converted to adipocytes in the presence of an adipogenic cocktail. In this study, the effects of DSW on differentiation adipocyte 3T3-L1 cells were studied. DSW significantly decreased lipid accumulation, a marker of adipogenesis, in a dose-dependent manner. DSW of hardness 1,000 was the most effective for inhibiting adipocyte differentiation without any cytotoxicity. DSW significantly reduced expression mRNA levels of PPARγ and C/EBPα and protein levels of fatty-acid-binding protein and adiponectin. Our results suggest a potential role for DSW as anti-obesity agents by inhibiting adipocyte differentiation mediated through the down-regulated expression of adipogenic transcription factors and adipocyte-specific proteins.
KeywordsAdipocytes Adipogenesis Deep-sea water C/EBPα PPARγ
This research was supported by the Korea Institute of Industrial Technology Evaluation and Planning Grant funded by Ministry of Commerce, Industry, and Energy of Korea (2008, Regional Innovation System).
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